摘要
鰤鱼诺卡氏菌(Nocardia seriolae)为鱼类诺卡氏菌病的主要病原,绛红色诺卡氏菌(N.purpurea)是分离自土壤中一种无毒诺卡氏菌。以鰤鱼诺卡氏菌和绛红色诺卡氏菌为亲本进行原生质体融合,获取融合菌。经生长特征观察、革兰染色、16SrRNA测序分析,对融合菌进行初步鉴定,并通过斑马鱼模型评估融合菌毒力检测和免疫保护效果。结果表明,融合菌的菌落形态与亲本菌株不同,为诺卡氏菌属革兰阳性菌,融合菌的毒力较鰤鱼诺卡氏菌野生株降低70%,免疫保护率约为50%。
Nocardia seriolae,the main pathogen of fish nocardiosis,and N.purpurea,a non-toxic Nocardia sp.isolated from soil,were selected as parent strains for protoplast fusion.The fusion strains were identified by colony morphology,Gram staining and16s rRNA sequencing.Then the virulence test and immune protective effects of the fusion strains were evaluated by the zebrafish model.The results showed that the fusant was Gram-positive nocardia bacteria,and its colony morphology was different from the parent strains.The virulence of fusant decreased70%compared with wild strains N.seriolae ZJ0503and the immune protection rate of fusant is about50%.
作者
陈建林
严聪羚
张红莲
苏泽杰
夏立群
CHEN Jian-lin;YAN Cong-ling;ZHANG Hong-lian;SU Ze-jie;XIA Li-qun(Fisheries College of Guangdong Ocean University;Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals;Key Laboratory of Diseases Controlling for Aquatic Economic Animals of Guangdong Higher Education Institutions, Zhanjiang 524088, China)
出处
《广东海洋大学学报》
CAS
2017年第3期38-42,共5页
Journal of Guangdong Ocean University
基金
广东省科技发展专项资金项目(2016A050502061)
广东省自然科学基金(2014A030313602)
深圳大鹏新区产业发展专项资金项目(KY20160207)
广东省攀登计划项目(pdjh2016b0234)