摘要
熟性是马铃薯的重要数量性状之一。本研究以马铃薯早熟品种中薯3号和晚熟品种中薯19及其熟性分离群体(221份)为材料,高通量简化基因组测序和集群分离分析(BSA)相结合,开发获得一个SCAR标记,命名为SCAR5-8。进一步利用该标记对分离群体的53份早熟和63份晚熟子代及70份四倍体马铃薯品种进行验证,结果表明,该标记在分离群体和四倍体品种中的检测结果与表型鉴定结果总体吻合度分别达87.1%与81.4%,Pearson’s双侧相关分析显示,其相关性均达极显著水平。该SCAR标记可被用于标记辅助选择,对加速四倍体马铃薯育种进程具有重要意义。
Maturity is one of the most important quantitative traits in potato.In this study,a maturity linked molecular markerSCAR5-8was developed using Zhongshu3,Zhongshu19and their F1segregation population consisting of221individuals viareduced-representation sequencing and bulked segregant analysis.A further testing of marker SCAR5-8was performed using53early maturity individuals,63late maturity individuals and70tetraploid varieties,showing the selection accuracy87.1%and81.4%between marker test and phenotyping in segregation population and tetraploid varieties,respectively.Moreover,Pearson’scorrelation analysis also showed that the marker SCAR5-8was significantly correlated to maturity trait in potato.The developedSCAR5-8marker could be used in marker-assisted selection.
作者
李兴翠
李广存
徐建飞
段绍光
卞春松
庞万福
刘杰
金黎平
LI Xing-Cui;LI Guang-Cun;XU Jian-Fei;DUAN Shao-Guang;BIAN Chun-Song;PANG Wan-Fu;LIU Jie;JIN Li-Ping(Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences / Key Laboratory of Biology and Genetic Improvement of Tuber and Root Crops, Ministry of Agriculture, Beijing 100081, China)
出处
《作物学报》
CAS
CSCD
北大核心
2017年第6期821-828,共8页
Acta Agronomica Sinica
基金
国家高技术研究发展计划(863计划)项目(2013AA102603)
国家现代农业产业技术体系建设专项(CARS-10)资助~~
关键词
马铃薯
熟性
标记开发
分子标记辅助育种
Potato
Maturity
Marker development
Marker-assisted selection
