氨基修饰聚醚醚酮表面及其成骨细胞相容性

Surface Osteoblast Compatibility of Amino-modified Polyetheretherketone

首先采用硼氢化钠将聚醚醚酮(PEEK)表面的羰基还原为羟基,获得羟基化预处理的PEEK,再用3-氨基丙基三乙氧基硅烷与其进行反应,将氨基(—NH2)引入至PEEK表面以改善其细胞相容性。用X射线光电子能谱和力学性能测试对表面化学处理的PEEK进行表征,同时,用MC3T3–E1成骨细胞评价表面氨基修饰的PEEK的细胞相容性。结果表明,硼氢化钠成功地将PEEK表面的羰基还原为羟基,—NH2成功地引入至PEEK表面;表面化学处理前后PEEK的拉伸及弯曲强度未发生明显变化;和未修饰的PEEK表面相比,—NH2修饰的PEEK表面能显著促进成骨细胞的粘附、铺展及增殖。

Polyetheretherketone(PEEK)surface activation of carbonyl being reduced to hydroxyl was done with sodium borohydride.And then treatment of the hydroxylation-pretreated PEEK samples with a substituted3-aminopropyltriethoxysilane solutionendowed PEEK surface with amino(–NH2)to enhance the cytocompatibility of PEEK surface.X-ray photoelectron spectroscopy andmechanical properties tests were employed to characterize the surface chemical modified PEEK samples.Meanwhile,MC3T3–E1osteoblast was used to evaluate in vitro biocompatibility of amino-modified PEEK surface.The results show that the carbonyl groupson PEEK surface are successfully reduced to hydroxyl groups by sodium borohydride and-NH2is successfully introduced on PEEKsurface.Surface modifications do not affect the tensile strength and flexural strength of PEEK.MC3T3–E1osteoblast adhesion,spreading and proliferation on amino-modified PEEK surface are remarkably improved compared to intact PEEK.