喹啉降解菌Rhodococcus sp.的降解特性与生物强化作用

Bioaugmentation and characteristics of a quinoline-degrading strain Rhodococcus sp.

以喹啉为唯一碳氮源从某焦化废水处理厂活性污泥中筛选出一株喹啉降解菌菌株红球菌(Rhodococcus sp.)KDQ2,其在24h内能将400mg/L喹啉降解96%,降解的最适条件为37℃和pH6~9,降解动力学符合Haldane方程.KDQ2能利用吡啶,但不能利用苯酚;在喹啉、吡啶和苯酚共存条件下,150mg/L吡啶和400mg/L苯酚不影响150mg/L喹啉在1d时的降解效率.KDQ2能适应含有高浓度苯酚、吡啶和喹啉等污染物的焦化废水环境条件,可以与活性污泥中的微生物共存,提高实际焦化废水中喹啉和TOC的去除能力.

The bacterial strain Rhodococcus sp.KDQ2capable of utilizing quinoline as sole carbon and nitrogen source,and energy was isolated from the activated sludge of a coking wastewater treatment plant.The optimum temperature andinitial pH for quinoline degradation were determined to be37℃and6~9,respectively.KDQ2degraded96%of quinolineat a200mg/L initial concentration within24h,and its degradation kinetics could be described with Haldane’s model.KDQ2was shown to be able to also utilize pyridine but not phenol.The removal of quinoline(150mg/L)was not inhibitedby the presence of pyridine(150mg/L)and phenol(400mg/L)in1d.KDQ2was able to adapt to real coking wastewatercontaining high concentrations of quinoline,pyridine and phenol,KDQ2coexisted with other microbes of activatedsludge in aeration tank and notably improved the removal of quinoline and TOC.