高分辨率熔解曲线法在GLP1R基因多态性位点rs3765467检测中的应用

Application of High Resolution Melting Method in the Detection of GLP1R Gene Polymorphism Site rs3765467

目的:建立检测肠促胰岛素样肽-1受体(GLP1R)基因已知突变位点rs3765467(NT_007592.16的第39 065 819位)的方法,并评价其准确性与实用性。方法:收集我院体检中心2015年10月-2016年2月72例健康体检者的外周静脉血样本,采用柱提法提取其全血DNA,经降落聚合酶链反应扩增后,采用高分辨率熔解曲线(HRM)法对产物进行分析;同时选取其中38例受试样本进行双脱氧链终止法(Sanger测序法)测序验证,比较2种方法的结果。结果:突变扫描结果显示,扩增片段中存在39 065 817和39 065 819两个多态性位点。HRM法只检测出了4种基因型[GCG/GCG、GCA/GCG或ACG/GCG、GCA/GCA或ACG/ACG、A(G)CA(G)];而Sanger测序法共检测出6种基因型[GCG/GCG、ACG/GCG、ACG/ACG、A(G)CA(G)、GCA/GCG、GCA/GCA]。结论:HRM法可区分GCG/GCG和A(G)CA(G)基因型,但无法区分GCA/GCG与ACG/GCG杂合突变、GCA/GCA与ACG/ACG纯合突变。该方法并不适用于多个邻近位点的单核苷酸多态性检测。在进行单核苷酸突变检测时,应对序列进行综合分析后再选取经济、简便的方法。

OBJECTIVE:To establish the method for the detection of the known glucagon-like peptide1receptor(GLP1R)gene mutation site rs3765467(NT_007592.16,position:39065819),and to evaluate its accuracy and practicability.METHODS:Peripheral venous blood samples of72healthy subjects were collected in medical examination center of our hospital during Oct.2015-Feb.2016.The whole blood DNA was extracted by column extraction method.After amplified by touch down PCR,high resolutionmelting(HRM)method was adopted to analyze amplified product.Sequencing verification by double stranded chain terminationmethod(Sanger sequencing method)was performed for38test samples.The results of2methods were compared.RESULTS:The results of mutation scanning showed that there were39065817and39065819polymorphism sites in amplified segments.Four types of mutations were detected by HRM method[GCG/GCG,GCA/GCG or ACG/GCG,GCA/GCA or ACG/ACG,A(G)CA(G)],but6types of mutations was detected by Sanger sequencing method[GCG/GCG,ACG/GCG,ACG/ACG,A(G)CA(G),GCA/GCG,GCA/GCA].CONCLUSIONS:HRM method can identify GCG/GCG and A(G)CA(G)genotype,but can notidentify GCA/GCG and ACG/GCG heterozygous mutation,GCA/GCA and ACG/ACG homozygous mutation.The method is notsuitable for the detection of single nucleotide polymorphism for multiple neighboring sites.In the detection of single nucleotide mutation,economical and simple method should be selected after comprehensive analysis of sequence.