芍药查尔酮异构酶基因CHI的表达特性分析

Expression pattern analysis on the chalcone isomerase (CHI) gene in Paeonia lactiflora cultivars

为了揭示查尔酮异构酶基因(chalcone isomerase gene,CHI)在芍药花瓣中的表达规律与特点,以不同芍药托桂型品种(‘金辉’‘彤云金焰’‘红楼锦菊’)4个不同发育时期(花蕾期、初开期、盛开期、衰败期)中的内、外花瓣为对象,用qPCR分别检测CHI基因的表达水平,分析其在不同芍药品种、不同发育时期以及内外花瓣之间的表达差异。结果显示:不同发育时期同一品种芍药花瓣组织CHI基因的表达量存在一定差异,从花蕾期到衰败期整体表现为上升趋势;不同品种同一发育时期‘彤云金焰’花瓣组织(内瓣和外瓣)CHI基因的表达水平明显高于‘金辉’和‘红楼锦菊’的;同一芍药品种在相同发育时期内瓣组织的CHI基因表达量均明显高于外瓣组织的。鉴于不同芍药品种、不同发育时期内外花瓣间颜色存在差异,推测CHI基因表达量可能与芍药花瓣颜色的形成有关。该结果可为深入研究芍药CHI功能并开展芍药花色遗传改良分子育种研究提供技术支撑。

Chalcone isomerase(CHI)is a key gene in regulating the formation of yellow petals.Inner and outer petalsfrom different Paeonia lactiflora cultivars including Jinhui,Tongyunjinyan,Hongloujinju at four growing stages(flower–bud stage,initiating bloom stage,bloom stage,wither stage)were selected as materials to study expressionpattern and characteristic of CHI gene.Expression difference of CHI gene in inner and outer petal at four growing stagesamong different Paeonia lactiflora cultivars were conducted using qPCR technology.The results showed that theexpression level of CHI gene exhibited difference in petal tissues from the same cultivar and showed a rise trend fromflower bud stage to wither stage;For different cultivars,the expression level of CHI gene in petal tissues ofTongyunjinyan was apparently higher than that of Jinhui and Tongyunjinyan at the same growing stages,furthermore,itwas apparently higher in inner petals than that in outer petals for the same cultivars at same growing stages.Consideringcolor difference in inner and outer petals at different growing stages and different Paeonia lactiflora cultivars,it wasspeculated that the expression level of CHI gene might be related to the color formation of petals in Paeonia lactiflora.The result could provide theoretical guidance for further research on the CHI function and genetic improvement to flowercolor of Paeonia lactiflora.