温度与盐度对云龙石斑鱼幼鱼耗氧率和排氨率的影响

Effects of temperature and salinity on oxygen consumption rate and ammonia excretion rate of junevile Epinephelus groupers

为了研究饱食和饥饿状态下温度(24、27、30和33℃)、盐度(15、20、25、30和33)对云龙石斑鱼幼鱼(Epinephelus groupers)耗氧率(OCR)和排氨率(AER)的影响,采用密闭流水式呼吸实验方法对OCR和AER进行了测定。结果显示:饱食和饥饿状态下,温度、盐度对云龙石斑鱼幼鱼OCR和AER影响均显著(P<0.05);当温度为24℃~33℃时,饱食状态下的OCR和AER分别比饥饿状态的提高30.29%~131.84%和131.82%~316.19%。饱食状态下,氧氮比(O∶N)、蛋白质供能比(PSR)、Q10(呼吸)、Q10(代泄)分别为12.54~20.18、27.74%~48.48%、1.21、1.60;而在饥饿状态下分别为25.77~35.90、19.49%~27.19%、1.87、3.41;当盐度为15~33时,饱食状态下OCR和AER分别比饥饿状态的提高100.41%~128.21%、182.19%~382.83%。饱食状态下,O∶N、PSR分别为13.32~20.72、39.47%~49.42%,而在饥饿状态下分别为29.18~30.98、22.59%~23.98%。研究表明,云龙石斑鱼幼鱼的最适生长温度为24℃~27℃,最适生长盐度为30~33。

In order to study the effects of temperature(24,27,30and33℃)and salinity(15,20,25,30and33)on oxygen consumption rate(OCR)and ammonia excretion rate(AER)of juvenile Epinephelus groupers in repletion and hunger,the OCR and AER were examined with the method of closed flow respiration experiment.The results showed that both in repletion and hunger,the OCR and AER of juvenile Epinephelus groupers were affected by temperature and salinity significantly(P<0.05);when the temperature was24℃-33℃,the OCR and AER in repletion were respectively30.29%-131.84%and131.82%-316.19%more than in hunger.In repletion,O∶N,Protein-supplying ratio(PSR),Q10(respiration)and Q10(excretion)were12.54-20.18,27.74%-48.48%,1.21and1.60respectively,and in hunger,25.77-35.90,19.49%-27.19%,1.87and3.41respectively;when the salinity was15-33,the OCR and AER in repletion were respectively100.41%-128.21%and182.19%-382.83%more than in hunger.In repletion,O∶N and PSR were13.32-20.72and39.47%-49.42%respectively,and in hunger,29.18-30.98and22.59%-23.98%respectively.The study showed that the optimum growth temperature of juvenile Epinephelus groupers was24℃-27℃,and the optimum growth salinity was30-33.