子宫内膜异位症纤维化与M2型巨噬细胞的关系

Relationship between fibrosis and M2 macrophages in endometriosis

目的:观察子宫内膜异位症模型小鼠纤维化程度与M2型巨噬细胞的关系。方法 :(1)用腹腔注射法建立BALB/c小鼠子宫内膜异位症模型,在造模第7、14、21天观察小鼠异位病灶的生长情况;(2)Masson染色法观察异位病灶、在位内膜的纤维化程度;(3)免疫组化法测定CD206、CD68的表达;(4)应用图像分析系统Image-Pro-Plus6.0(IPP6.0)对染色结果进行半定量分析,探讨纤维化程度与M2型巨噬细胞表达的相关性。结果:(1)腹腔注射法建立子宫内膜异位症模型成功率为100%;(2)子宫内膜异位症模型小鼠纤维化程度与M2型巨噬细胞表达呈正相关。结论:腹腔注射法可以成功建立子宫内膜异位症小鼠模型。M2型巨噬细胞可能促进子宫内膜异位症纤维化。

Objective To investigate the relationship between fibrosis and M2macrophages in endometriosis.Methods BALB/c mice model of endometriosis was established by intraperitoneal injection.The growth of ectopiclesions in mice was observed on the7th,14th and21th day after modeling.Masson staining was used to observe thedegree of fibrosis and immunohistochemical method to detect the expression of CD206and CD68of mice.Image?Pro?Plus6.0was used for semi quantitative analysis of staining and the correlation between the degree of fibrosis andthe expression of M2macrophages was explored.Results The success rate of the establishment of endometriosismodel by intraperitoneal injection was100%.There was a positive correlation between fibrosis and the expression ofM2macrophages in endometriosis mice model.Conclusions The mice model of endometriosis can be establishedsuccessfully by intraperitoneal injection and M2macrophages may promote fibrosis in endometriosis.