银杏叶提取物对慢性阻塞性肺疾病大鼠CRP、TNF.α的影响

Effects of ginkgo biloba extract on CRP and TNF-α in rats with chronic obstructive pulmonary disease

目的:探讨银杏叶提取物(GBE)对慢性阻塞性肺疾病(COPD)大鼠血清、肺泡灌洗液(BALF)中C反应蛋白(CRP)及肿瘤坏死因子-α(TNF-α)的影响。方法:90只大鼠随机分为正常对照组(A组)、COPD对照组(B组)、GBE早期干预组(C组)、GBE后期干预组(D组)、红霉素早期干预组(E组)、红霉素后期干预组(F组),每组15只。除A组外,余各组进行28 d COPD造模。C、D组在第1~14天和29~42天用GBE干预;E、F组在第1~14天和第29~42天用红霉素干预,实验结束后检测各组大鼠血清及BALF中CRP、TNF-α的含量。结果:与B组比较,C、D、E、F组血清及BALF中CRP、TNF-α含量均显著下降(P<0.05);C、E、F组血清及BALF中CRP、BALF中TNF-α含量下降较D组明显(P<0.05)。结论:GBE可抑制COPD大鼠气道及全身炎症反应,且早期干预效果更好。

Objective To study the effects of ginkgo biloba extract(GBE)on c?reactive protein(CRP)and tumor necrosis factor?α(TNF?α)in serum and alveolar lavage fluid(BALF)from rats with chronic obstructivepulmonary disease(COPD).Methods90rats were randomly divided into groups A,B,C,D,E and F.Therewere15rats in each group.The rat model of COPD were established in groups B,C,D,E and F.Groups C and Dwere given intraperitoneal injections with GBE from day l to day l4and day29to day42.Groups E and F were given intraperitoneal injections with erythromycin from day l to day l4and day29to day42.After the end of experi?ment,the contents of CRP and TNF?αin serum and BALF were detected in all groups.Results The contentsof CRP and TNF?αin the serum and the BALF were markedly lower in groups C,D,E and F than in group B(P<0.05);and the contents of CRP in the serum and the BALF and TNF?αin the BALF were lower in groups C,E and F than in group D(P<0.05).Conclusions GBE can inhibit the airway and systemic inflammatory responsein COPD rats.Early intervention is more effective.