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乌鳢出血病的病原及组织病理学研究 被引量:5

Isolation, Identification and Pathohistological Observation of Channa argus Infected by Aeromonas sobria
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摘要 对广东顺德勒流镇某水产养殖场患出血病的乌鳢进行病原分离,对分离菌进行形态学分析、生化鉴定、PCR鉴定和人工感染。经鉴定,分离菌为温和气单胞菌,其半致死温度LD50为1.645×105 CFU/g。组织病理学观察表明,鳃小片毛细血管严重充血,基部细胞增生,部分鳃小片相互融合成肉芽肿结构;肝细胞出现空泡,部分区域肝细胞坏死、溶解和脂肪变性;肾小体血管球充血破裂,部分肾小管上皮细胞玻璃样变,细胞质中出现大量空泡;脾脏中血细胞明显增多,淋巴细胞减少;肠黏膜下层和固有膜可见结缔组织增生、水肿现象。温和气单胞菌可引起乌鳢的肝、脾和肾等重要器官出现典型的病理变化并引起死亡。 The pathogen was isolated from Channa argus from a farm in Foshan,Guangdong province.The isolated bacterium was identified by morphological characteristics,biochemical characteristicsidentification,amplification of16s rRNA by PCR and experimental infection test.The pathogen wasconfirmed as Aeromonas sobria and the value of LD50was1.645×105CFU/g.Histopathologicalobservation of the diseased fish organs showed that the basal cells of the gill lamellae were hyperplasia,severely fused with each other to form granuloma.The hepar cells were seriously vacuolar and somecells showed necrosis,dissolution and steatosis.Glomerular vessels of renal corpuscle ruptured,and partof the renal tubular epithelial cell showed vitreous changes,some vacuoles appeared in the cytoplasm ofthe renal tubular.The blood cells increased significantly and lymphocytes decreased in the spleen.Thevisible tissue hyperplasia and edema appeared in the intestinal submucosal and intrinsic membrane.Theanalysis of histopathology indicated that A.sobria can cause typical pathological changes in the liver,spleen and kidney of C.argus.
作者 伍元植 简纪常 蔡双虎 WU Yuan-zhi;JIAN Ji-chang;CAI Shuang-hu(Fisheries College of Guangdong Ocean University, Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals & Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Zhanjiang 524088, China)
出处 《广东海洋大学学报》 CAS 2017年第4期41-45,共5页 Journal of Guangdong Ocean University
基金 广东省科技计划(2013B020307015)
关键词 乌鳢 出血病 病原 温和气单胞菌 病理观察 Channa argus hemorrhage pathogen Aeromonas sobria pathohistological observation
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