摘要
以带叶兜兰(Paphiopedilum hirsutissimum)组培苗为外植体,研究5种培养基对带叶兜兰组培苗壮苗生根的影响,设计6种基质处理,以筛选组培苗出瓶后的最佳移栽基质。结果表明:初步筛选出较适宜壮苗生根的培养基为1/2MS+NAH2PO4170 mg/L+NAA 0.1 mg/L,植株生物量增加显著;随着NAA浓度的增加,植株的壮苗生根效果受到不同程度的抑制。单独利用腐熟过的碎树皮做基质,试管苗移栽成活率均达99.7%以上。
Taking tissue culture seedling ofPaphiopedilum hirsutissimum as tested material,the effects of five different mediums on P.hirsutissimum seedling growth and rooting were investigated,and six different transplanting matrix treatments were designed to select the best one for the transplanting of tissue culture seedling.The results showed that1/2MS+NAH2PO4170mg/L+NAA0.1mg/L was the optimum medium for the seedling growth and rooting,and the plant biomass increased significantly.With the increase of NAA concentration,plant seedling rooting was inhibited.The maturity of the crushed bark was the optimized matrix for thein vitro plantlet ofP.hirsutissimum transplantation,which produced higher survival rate(>99.7%).
作者
陈宝玲
周千淞
陈尔
王华新
龚建英
龙定建
唐庆
CHEN Bao-ling;ZHOU Qian-song;CHEN Er;WANG Hua-xin;GONG Jian-ying;LONG Ding-jian;TANG Qing(Guangxi Forestry Research Institute,Nanning 530002,China;Guangxi State Qipo Forest Farm,Nanning 530225,China)
出处
《广西林业科学》
2017年第2期206-209,共4页
Guangxi Forestry Science
基金
广西自然科学基金项目(2015GXNSFBA139053)
广西林业科技项目(桂林科研[2015]第39号)
关键词
带叶兜兰
离体培养
生根
基质
Paphiopedilum hirsutissimum
isolated culture
seedling rooting
matrix
