摘要
目的探讨茶黄素对小鼠胸腺放射性损伤的防护作用。方法 25只C57BL/6小鼠随机分为对照组、照射组、照射+25mg/kg茶黄素组、照射+50mg/kg茶黄素组、照射+100mg/kg茶黄素组。对照组小鼠给予0Gy照射,其余小鼠接受4Gy全身照射。照射后第14天取小鼠胸腺检测胸腺指数、胸腺细胞总数,以确定茶黄素的最佳给药剂量。另取32只C57BL/6小鼠,根据最佳给药剂量将其随机分为对照组、茶黄素组、照射组、照射+茶黄素组,照射组和照射+茶黄素组小鼠接受4Gy的全身照射。照射后第14天检测胸腺组织形态、CD4CD8 T细胞亚群和胸腺细胞活性氧(ROS)水平。结果受辐射小鼠胸腺有放射性损伤,表现为胸腺指数下降,胸腺细胞总数减少,胸腺组织形态改变,胸腺T细胞亚群改变及胸腺细胞ROS水平升高。与照射组相比,照射+50mg/kg茶黄素组小鼠胸腺指数和胸腺细胞总数升高(P<0.05),且照射+50mg/kg茶黄素组小鼠胸腺细胞总数高于照射+25mg/kg茶黄素组(P<0.05)。与照射组相比,照射+茶黄素组胸腺病理改变改善,胸腺CD4^+CD8^-、CD4^+CD8^+细胞比例升高,而CD4^-CD8^-细胞比例下降(P<0.05),胸腺细胞内ROS水平降低(P<0.05)。结论茶黄素对4Gy全身照射诱导的小鼠胸腺放射性损伤具有一定的保护作用。
Objective To investigate the protective effect of theaflavins on thymus injury caused by total body irradiation(TBI).Methods Twenty-five C57BL/6mice were randomly divided into5groups:control group,4Gy TBI group,4Gy TBI+25mg/kg theaflavins group,4Gy TBI+50mg/kg theaflavins group and4Gy TBI+100mg/kg theaflavins group.Thymus index and total number of thymocytes were detected at the14th d post-irradiation to determine the optimal dose of theaflavins.According to this optimal dose,32C57BL/6mice were randomly divided into4groups:control group,theaflavins group,4Gy TBI group and4Gy TBI+theaflavins group.Thymus histomorphology,CD4CD8T cell subsets,and reactive oxygen species(ROS)in thymocytes were examined at the14th d post-irradiation.Results The irradiated thymus exhibited decreased thymus index and total number of thymocytes(P<0.05),aberrant histomorphology and T cell subsets(P<0.05),and increased ROS level in thymocytes(P<0.05).Compared with4Gy TBI group,the thymus index and total number of thymocytes were significantly increased in4Gy TBI+50mg/kg theaflavins group(P<0.05).The total number of thymocytes was significantly higher in4Gy TBI+50mg/kg theaflavins group than that in4Gy TBI+25mg/kg theaflavins group(P<0.05).Therefore,50mg/kg theaflavins was chosen as the optimal dose for subsequent experiments.Moreover,the aberrant histomorphology of irradiated thymus was alleviated by theaflavins.A decline in the percentage of CD4-CD8-T cells and an elevation of CD4+CD8-and CD4+CD8+T cells were found in irradiated mice administered with theaflavins(P<0.05).Compared with4Gy TBI group,the ROS level was significantly decreased in4Gy TBI+theaflavins group(P<0.05).Conclusion Theaflavins exhibits a protective effect on radiation-induced thymus injury.
作者
韩晓丹
张俊伶
薛晓蕾
赵玉
樊赛军
HAN Xiao-dan;ZHANG Jun-ling;XUE Xiao-lei;ZHAO Yu;FAN Sai-jun(Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Science and Peking Union Medical College, Tianjin 300192, China)
出处
《天津医药》
CAS
2017年第7期699-703,共5页
Tianjin Medical Journal
基金
国家自然科学基金资助项目(81402633
81572969
81402541)
科技部科研院所技术发展研究专项(2014EG150134)
中国医学科学院医学与健康创新工程重大项目(2016-I2M-1-017)
天津科技支撑计划项目(14ZCZDSY00001)
天津市自然科学基金青年项目(16JCQNJC13600)
关键词
胸腺
辐射损伤
活性氧
小鼠
近交C57BL
茶黄素
免疫功能
thymus gland
radiation injuries
reactive oxygen species
mice, inbred C57BL
theaflavins
immune function