子宫内膜异位症患者腹腔液单个核细胞IFN-γ及IL-10mRNA的表达及意义

Expressions and significance of IFN-γ and IL-10 mRNA in peritoneal fluid mononuclear cells of patients with endometriosis

目的探讨子宫内膜异位症(EM)患者腹腔液单个核细胞(PFMC)γ干扰素(IFN-γ)及白细胞介素(IL)-10 mRNA的表达水平及其意义。方法选取经手术后病理证实为EM的患者55例作为EM组,以非EM患者52例作为对照组。分离PFMC,应用Taq Man实时荧光逆转录聚合酶链反应(RT-PCR)测定IFN-γ及IL-10 mRNA的表达水平。结果 EM组PFMC IFN-γ mRNA的表达水平低于对照组,而IL-10 mRNA的表达水平明显高于对照组(P<0.05),EM组IFN-γ/IL-10比值较对照组明显降低(P<0.05);Ⅰ~Ⅱ期EM患者IFN-γ/IL-10比值高于Ⅲ~Ⅳ期患者,差异有统计学意义(P<0.05)。结论 EM患者PFMC中IFN-γ/IL-10失衡可能与EM的发生发展有关。

Objective To investigate the expression levels and significance of interferon-γ(IFN-γ),interleukin(IL)-10mRNA in peritoneal fluid mononuclear cells(PFMC)in patients with endometriosis(EM).Methods A total of55patients with EM diagnosed histopathologically after operation were enrolled as EM group,and52patients without EM were enrolled as control group.The PFMC were isolated,and the expression levels of IFN-γand IL-10mRNA were determined by TaqMan real-time reverse transcriptase-polymerase chain reaction(RT-PCR).Results It was found that IFN-γmRNA expression level was significantly decreased in EM group compared with that in control group(P<0.05),while IL-10mRNA expression level was significantly increased in EM group compared with that in control group(P<0.05).The ratio of IFN-γ/IL-10was significantly lower in EM group than that in control group(P<0.05).The ratio of IFN-γ/IL-10was significantly higher in patients with stageⅠ-Ⅱthan that in patients with stageⅢ-Ⅳ(P<0.05).Conclusion The imbalance of IFN-γ/IL-10in PFMC is associated with the pathogenesis of EM.