云南松松塔提取物对小鼠急性酒精性肝损伤的影响

Effects of Pinus Yunnanensis on Acute Alcoholic Liver Injury in Mice

目的观察云南松松塔提取物对小鼠急性酒精性肝损伤的影响,探讨其作用机制。方法采用酒精灌胃制备急性酒精性肝损伤小鼠模型。实验小鼠随机分为正常组、模型组、阳性对照组和云南松松塔低、中、高剂量组,各给药组给予相应药物灌胃,每日1次,连续7 d。末次灌胃3 h后,计算肝、脾指数,检测丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、丙二醛(MDA)、一氧化氮(NO)含量,超氧化物歧化酶(SOD)、微量还原型谷胱甘肽(GSH)活性,HE染色观察肝脏病理形态。结果与模型组比较,云南松松塔各剂量组小鼠肝指数显著降低(P<0.01),云南松松塔高剂量组脾指数显著降低(P<0.01),云南松松塔中、高剂量组血清AST含量显著降低(P<0.01),云南松松塔各剂量组肝组织GSH活性显著升高(P<0.05,P<0.01);云南松松塔低、高剂量组肝组织MDA含量显著降低(P<0.05);云南松松塔各剂量组血清ALT含量降低,肝组织SOD活性升高和NO含量降低,差异均无统计学意义(P>0.05);HE染色结果显示,云南松松塔各剂量组小鼠肝组织损伤较模型组明显改善。结论云南松松塔提取物对小鼠急性酒精性肝损伤具有保护作用。

Objective To study the effect of Pinus yunnanensis on acute alcoholic liver injury in rats and explore its mechanism.Methods A model of acute alcoholic liver injury in mice was prepared by alcohol.The mice were randomly divided into normal control group,model group,positive control group,Pinus yunnanensis low-,mediumand high-dose groups.Mice in the medicine group were given the corresponding medicine by gavage once a day for7days.After the last three hours of intragastric administration,the liver and spleen index,ALT,AST and GSH in serum,SOD,MDA and NO in liver homogenates were measured.Histopathological changes of liver were observed by HE staining.Results Compared with model group,Pinecone of Pinus unnanensis high-,medium-and low-dose groups could significantly reduce the liver index in mice(P<0.01),and high dose groups could significantly reduce the number of spleen(P<0.01);The contents of AST in the medium-and high-dose groups significantly decreased(P<0.01)and the GSH activity significantly increased(P<0.05,P<0.01).There was no significant difference in serum ALT level,SOD activity,GSH activity and NO content in the liver tissues of Pinus yunnanensis groups(P>0.05).HE staining results showed that,the damage of liver tissue in mice of Pinus yunnanensis was significantly improved compared with the model group.Conclusion Pinus yunnanensis has protective effects on acute alcoholic liver injury in mice.