基于一测多评法测定甘肃红芪中4种黄酮类成分

Content Determination of Four Flavonoids in Hedysari Radix in Gansu Province Based on Quantitative Analysis of Multi-components by Single Marker

目的建立甘肃红芪中芒柄花苷、毛蕊异黄酮、金雀异黄酮、芒柄花素4种黄酮类成分的一测多评法,验证该方法在红芪质量评价中应用的准确性及可行性。方法以毛蕊异黄酮为内标,分别建立毛蕊异黄酮与芒柄花苷、金雀异黄酮、芒柄花素的相对校正因子,计算红芪中毛蕊异黄酮、金雀异黄酮、芒柄花素的含量,实现一测多评。结果各相对校正因子重复性良好,一测多评法测定结果与外标法无显著差异。结论以毛蕊异黄酮为内标,同时测定芒柄花苷、金雀异黄酮、芒柄花素的一测多评法可用于红芪的定量分析。

Objective To establish a method for simultaneous determination for the contents of four flavones(ononin,calycosin,genistein and formononetin)of Hedysari Radix in Gansu Province with quantitative analysis of multi-components by single-marker(QAMS);To prove its feasibility and accuracy.Methods Calycosin was taken as internal standard substance.Relative correction factors(RCF)of ononin,genistein and formononetin to calycosin were established.The contents of ononin,calycosin,genistein and formononetin were determined to realize QAMS.Results RCF was with good repeatability.The results of QAMS were consistent with the results of the externalstandard method.Conclusion The method that determines the contents of ononin,genistein and formononetin with calycosin as internal standard substance,can be used for quantitative analysis of Hedysari Radix.