连翘、防风和辛夷混合挥发油提取及包合工艺研究

Study on Extraction and Inclusion Processes of Mixed Volatile Oils from Forsythiae Fructus,Saposhnikoviae Radix and Magnoliae Flos

目的筛选连翘、防风和辛夷混合的挥发油提取及β-环糊精包合的最佳工艺。方法以混合挥发油收取量为指标,单因素试验考察混合挥发油的最优提取工艺;选用饱和水溶液法,以混合挥发油包合率为指标,正交试验设计考察混合挥发油与β-环糊精投料比、包合时间和包合温度对包合工艺的影响;通过X射线衍射法、红外光谱法以及扫描电镜法对包合物进行验证。结果混合挥发油提取最优工艺为:加10倍量水,浸泡3 h,粉碎成细粉,提取5 h。最佳包合工艺为:挥发油(m L)与β-环糊精(g)比例为1∶10,包合温度为50℃,包合时间为2 h。X射线衍射法、红外光谱法及扫描电镜均表明混合挥发油包合物构建相对稳定。结论优选的提取和包合工艺稳定可行,为进一步制剂开发提供了研究基础。

Objective To optimize the extraction process of mixed volatile oil from Forsythiae Fructus,Saposhnikoviae Radix and Magnoliae Flos and inclusion process ofβ-cyclodextrin(β-CD).Methods With yield ratio of volatile oil as evaluation index,single factor experiments were used to study the extraction process of volatile oil;saturated aqueous solution was used,with inclusion rate of volatile oil as index,and orthogonal design was adopted to examine effects of charge ratio of volatile oil andβ-CD,inclusion temperature and inclusion time on the inclusion process;X-ray scattering technique,infrared spectroscopy and scanning electron microscopy were used to characterize the inclusion compound.Results The optimum extraction process of volatile oil was soaking fine powder extracted5hours with10folds the amount of water.The optimum conditions of inclusion process were as follows:mixed ratio of volatile oil(mL)andβ-CD(g)was1:10;inclusion temperature was50℃;the inclusion time was2h.X-ray scattering technique,infrared spectroscopy and scanning electron microscopy proved the inclusion compound had been formed.Conclusion Optimum extraction and inclusion processes are stable and feasible,and can provide research foundation for further research and development of preparation.