反相液相色谱法测定苦参中苦参碱和氧化苦参碱

Determination of Matrine and Oxymatrine in Sophora Flavescens by Reverse Phase Liquid Chromatography

建立反相液相色谱法测定苦参中苦参碱和氧化苦参碱含量的方法。提取溶剂为乙醇,选择C18色谱柱,以甲醇–水–三乙胺溶液(55∶45∶0.2)为流动相,流量为0.8 mL/min,紫外检测器波长为210 nm。苦参碱和氧化苦参碱的质量浓度在10~200 mg/L范围内均与其色谱峰面积呈良好的线性,线性相关系数均大于0.999,检出限分别为0.05,0.1 mg/L。苦参碱和氧化苦参碱的加标回收率分别为98.47%,97.89%,测定结果的相对标准偏差分别为1.32%,1.08%(n=6)。该方法操作简便,干扰少,灵敏度高。

A method for the determination of matrine and oxymatrine in sophora flavescens by reversed phase liquid chromatography was established.The extraction solvent was ethanol,C18column was selected,methanol-water-three ethylamine solution(55∶45∶0.2)was used as the mobile phase with the flow rate of0.8mL/min,and the UV detector wavelength was210nm.The concentration of matrine and oxymatrine was linear with its chromatographic peak area in the range of10-200mg/L,the linear correlation coefficients were more than0.999,and the detection limits were0.05,0.1mg/L,respectively.The recovery rates of matrine and oxymatrine were98.47%,97.89%,and the relative standard deviations of determination results were1.32%and1.08%(n=6),respectively.The method has the advantages of simple operation,less interference and high sensibility.