儿童原发性肾病综合征肾组织瞬时受体电位阳离子通道蛋白6表达及意义

Expression of TRPC 6 in renal tissue and its significance in children with primary nephrotic syndrome

目的探讨原发性肾病综合征(PNS)患儿肾组织中瞬时受体电位阳离子通道蛋白6(TRPC6)的表达与足细胞损伤的关系及其临床意义。方法获取18例PNS患儿的肾组织,常规切片染色光镜观察肾脏组织病理改变,电镜观察足细胞的结构变化,分别用q PCR和免疫组化测定组织中TRPC6 m RNA和蛋白的表达,并将TRPC6 m RNA表达分别与血清白蛋白(Alb)、肌酐(Cr)、三酰甘油(TG)、胆固醇(Tch)、补体C3水平及24 h尿蛋白定量和评估的肾小球滤过率(e GFR)进行相关性分析。结果 PNS患儿肾组织中TRPC6蛋白表达较对照组上调,差异有统计学意义(P<0.05)。PNS患儿肾组织中TRPC6 m RNA的相对表达量与肾组织TRPC6蛋白表达呈显著正相关(r=0.508,P<0.05),与血清Alb、Cr、TG、Tch、补体C3、e GFR水平以及24 h尿蛋白定量无相关性(P>0.05)。结论 PNS患儿的病理类型仍以足细胞病变为主,其肾小球足细胞中TRPC6蛋白表达升高。TRPC6检测可能有助于足细胞病变诊断。

Objective To explore the relationship between the expression of transient receptor potential cation channel subfamily C member6(TRPC6)and podocyte injury in children with primary nephrotic syndrome(PNS)and its clinical significance.Methods The renal tissue of18children with PNS was obtained.The pathological changes of kidney were observed by routine section staining and light microscopy.The structural changes of podocyte were observed by electron microscope.The mRNA and protein expressions of TRPC6in tissues were determined by qPCR and immunohistochemistry,respectively.Further the correlation of TRPC6mRNA with serum levels of albumin(Alb),creatinine(Cr),triacylglycerol(TG),cholesterol(Tch),complement C3and24h urinary protein quantitation and estimated glomerular filtration rate(eGFR)were analyzed respectively.Results The expression of TRPC6protein in renal tissue of children with PNS was higher than that in the control group,and the difference was statistically different(P<0.05).The relative expression of TRPC6mRNA in renal tissue of children with PNS was positively related to the expression of TRPC6protein(r=0.508,P<0.05),but there was no correlation of expression of TRPC6mRNA with serum levels of Alb,Cr,TG,Tch,C3,eGFR and24h urinary protein quantitation(P>0.05).Conclusion The pathological types of PNS were mainly podocyte lesions,and the expression of TRPC6protein was increased in podocytes.TRPC6detection may be helpful in the diagnosis of podocyte lesions.