红掌2个SOC1基因的克隆、序列与表达分析

Cloning, sequence and expression analysis of two SOC1 genes from Anthurium

为了明确SOC1转录因子在红掌中的成员及作用机制,采用5'/3'末端快速扩增技术获得这2个SOC1基因的完整编码区,通过生物信息学软件分析这2个基因的相关信息,并结合荧光定量反转录-聚合酶链式反应验证这2个基因的表达模式。结果显示:获得的2个基因核苷酸长度分别为651 bp和642 bp,命名为Aa SOC1-1和Aa SOC1-2;2个转录因子在二级结构上均有螺旋、折叠和转角,未发现其他结构。系统进化分析显示,二者与单子叶植物同类蛋白距离较近,与红掌的植物学分类地位一致。表达分析结果显示,2个基因均在营养器官和生殖器官中表达,但表达水平略有差异。研究认为,从红掌中克隆的2个SOC1转录因子可能定位于线粒体而非细胞核中,二者在序列组成和表达模式上既有保守性也存在一定的差异性,预示它们在功能上可能也存在一定的区别。

SUPPRESSOR OF OVEREXPRESSION OF CO1(SOC1)is a flowering integration factor that widely existed inflowering plants,which is necessary in multiple flowering induction pathways,including vernalization,photoperiod,gibberellin,and other endogenous or exogenous environmental signals.Therefore,researchoftheeffectof SOC1onplantgrowthanddevelopmentcan reveal the plant flowering mechanism and direct molecular breeding.Anthurium is a kind of high?grade potted flower inthe worldwide.However,molecular biology research on this plant had just started.As a flowering integration factor,SOC1inAnthurium may be a key to deeply understand the flower conversion process.