荷移反应分光光度法测定三唑仑

Spectrophotometric Determination of Triazolam Based on Charge Transfer Reaction

本文提供了电子受体7,7,8,8-四氰基对苯醌二甲烷(TCNQ)与电子供体三唑仑在乙醇-丙酮-水介质中检测三唑仑的荷移分光光度法。实验显示,50℃下加热50分钟形成组成比为1∶1的荷移络合物,络合物的最大吸收波长为846nm,缔合常数为2.817 103,吉布斯自由能变化为-19.68kJ·mol^(-1)。在最佳条件下,三唑仑浓度在7.8~125 mg·mL^(-1)范围内符合朗伯-比尔定律,相关系数r=0.9991,最低检测限为0.8 mg·L^(-1)。当三唑仑的质量浓度为100 mg·L^(-1)时,测定结果的相对标准偏差(n=5)为0.73%。采用本方法测定片剂中的三唑仑含量时,回收率为99.2%。

The charge transfer reaction of7,7,8,8-tetracyanoquinodimethane(TCNQ)as an electronacceptor and triazolam as an electron donor were investigated by spectrophotometer in the medium ofethanol-acetone-water.A charge transfer complex with composition ratio of1∶1was formed at50℃for50min,its absorption maximum wavelength was846nm,the complex stability constant and Gibbs freeenergy changes were2.817103and-19.68kJ·mol-1,respectively.Under the optimized conditions,Beer'sLaw was obeyed in the range of7.8~125mg?mL-1of triazolam with correlation coefficient of0.9991,standard deviation was0.0389and detection limit was0.8mg·L-1.Precision of the method was tested at theconcentration level of100mg·L-1for5multiplicate determinations,and relative standard deviation was0.73%.The proposed method was applied to the determination of triazolam in its tablets,giving results inconsistency with the labeled value.Recovery of the method was tested by standard addition method andvalues of recovery was99.2%.