微小RNA-1246在乳腺癌患者中的临床意义

MicroRNA-1246 in patients with breast cancer

目的分析微小RNA(miRNA)-1246在乳腺癌患者血清、组织中的表达,并探讨血清miRNA-1246对乳腺癌的诊断价值。方法采用荧光定量聚合酶链反应(PCR)检测100例乳腺癌患者、40名正常对照者血清以及30例乳腺癌组织、15例癌旁正常乳腺组织中miRNA-1246的表达。结果乳腺癌患者血清miRNA-1246相对表达量明显高于正常对照者(P<0.05),组织miRNA-1246相对表达量与正常癌旁组织比较,差异无统计学意义(P>0.05)。血清miRNA-1246诊断乳腺癌的受试者工作特征(ROC)曲线下面积为0.904,敏感性和特异性分别为93%、75%。结论血清miRNA-1246诊断乳腺癌具有良好的敏感性和特异性,可以作为乳腺癌潜在的肿瘤生物标志物。

Objective To analyze the expression of microRNA(miRNA)-1246in sera and tissues among breast cancer patients,and to investigate the role of serum miRNA-1246in the diagnosis of breast cancer.Methods Fluorescence quantitation polymerase chain reaction(PCR)was used to determine the expression of miRNA-1246in sera of100breast cancer patients and40healthy controls,in30cases of breast cancer tissues and15cases of breast cancer adjacent tissues.Results The expression of miRNA-1246was higher in sera of breast cancer patients than that in sera of healthy controls(P<0.05).There was no statistical significance for miRNA-1246expression between breast cancer tissues and breast cancer adjacent tissues(P>0.05).Serum miRNA-1246had an area under receiver operating characteristic(ROC)curve of0.904,with sensitivity of93%and specificity of75%.Conclusions Serum miRNA-1246shows good sensitivity and specificity for diagnosing breast cancer,and can be used as a potential tumor marker.