哮喘患儿支气管肺泡灌洗液GM-CSF和MPO水平的变化及意义

Changes of granulocyte-macrophage colony-stimulating factor and myeloperoxidase levels in bronchoalveolar lavage fluid among asthmatic children

目的探讨哮喘患儿支气管肺泡灌洗液(BALF)粒细胞-巨噬细胞集落刺激因子(GM-CSF)和髓过氧化物酶(MPO)在哮喘发生、发展中的作用。方法收集29例哮喘患儿哮喘发作期和缓解期以及25例对照患儿的BALF样本,在显微镜下计数中性粒细胞(NEU)百分率,采用酶联免疫吸附试验检测GM-CSF和MPO水平。结果哮喘组哮喘发作期和缓解期及对照组BALF GM-CSF的水平分别为(164.8±34.0)、(129.5±30.4)和(123.0±25.7)ng/L,MPO水平分别为(25.1±9.7)、(15.5±4.4)和(13.8±3.5)ng/L,NEU百分率分别为(35.1±7.6)%、(26.3±6.2)%和(23.8±5.5)%。哮喘组哮喘发作期BALF GM-CSF和MPO水平及NEU百分率均高于哮喘缓解期和对照组(P<0.01),而哮喘组哮喘缓解期BALF GM-CSF和MPO水平及NEU百分率与对照组比较差异均无统计学意义(P>0.05)。哮喘组哮喘发作期GM-CSF水平与NEU百分率、MPO水平呈正相关(r值分别为0.504、0.665,P<0.01),NEU百分率与MPO水平呈正相关(r=0.669,P<0.01)。结论哮喘患儿BALF GM-CSF和MPO水平升高与疾病的发生、发展关系密切,BALF GM-CSF和MPO可作为评价和监测哮喘患儿病情的有效指标。

Objective To investigate the roles of granulocyte-macrophage colony-stimulating factor(GMCSF)and myeloperoxidase(MPO)in bronchoalveolar lavage fluid(BALF)among asthmatic children.Methods A total of29asthmatic children and25controls were enrolled.By microscopy,the percentage of neutrophil(NEU)was determined.The levels of GM-CSF and MPO were determined by enzyme-linked immunosorbent assay.Results The levels of GM-CSF in BALF were(164.8±34.0)ng/L in attack stage group,(129.5±30.4)ng/L in remission stage group and(123.0±25.7)ng/L in control group.The levels of MPO were(25.1±9.7),(15.5±4.4)and(13.8±3.5)ng/L in the3groups,respectively.The percentages of NEU were(35.1±7.6)%,(26.3±6.2)%and(23.8±5.5)%in the3groups,respectively.The levels of GM-CSF and MPO in BALF and the percentages of NEU were higher in attack stage group than those in remission stage and control groups(P<0.01),but there was no statistical significance between remission stage and control groups(P>0.05).GM-CSF level was correlated positively with MPO level and the percentage of NEU(r=0.665and0.504,P<0.01),and the percentage of NEU was correlated positively with MPO level(r=0.669,P<0.01)in attack stage group.Conclusions Both GM-CSF and MPO levels increase among asthmatic children,and they might be involved in the pathological process of asthma.GM-CSF and MPO in BALF may be indices for evaluating and monitoring the status of children with asthma.