氯化钙浓度对念珠菌溶血活性的影响

Influence of concentrations of CaCl_(2)on hemolytic activity of candida strains

目的通过将固定浓度的念珠菌菌悬液分别滴加到含有不同浓度CaCl_2的羊血培养基上培养,观察念珠菌溶血活性的差异,评估CaCl_2对念珠菌溶血活性的影响。方法选择25株念珠菌,其中包括21株临床分离株和4株标准株,配制4个不同浓度CaCl_2的羊血培养基,分别为0%、0.5%、1%和2.5%(g/m L),于37℃、5%体积分数CO_2培养。观察24 h、48 h、72 h的溶血反应并拍照、记录。结果念珠菌在不同浓度CaCl_2的羊血培养基中的溶血指数存在差异,差异具有统计学意义(F=24.929,P<0.001)。其中不含CaCl_2的溶血指数为2.749±0.103,含0.5%、1%和2.5%依次为2.247±0.079、2.013±0.092和2.150±0.066。结论不同浓度的CaCl_2会影响羊血培养基上念珠菌的溶血活性。

Objective To investigate the influence of different concentrations of CaCl2on the hemolytic activity of candida strains.Methods A total of25candida strains were selected,including21candida strains recovered from clinical specimens,and4reference strains of C.albicans(ATCC90028)(American Type Culture Collection,Manassas,VA,USA),C.glabrata(ATCC90030),C.krusei(ATCC6258),and C.tropicalis(ATCC13803)respectively.CaCl2was added at concentrations of0%,0.5%,1%and2.5g%(wt/vol).Plates were cultured at37℃in a5%CO2atmosphere for24?72h,and the hemolytic index(Hi)was analyzed.Results Comparsions of the hemolytic indices among the groups treated with0.5%CaCl2(Hi=2.247±0.079),1%CaCl2(Hi=2.013±0.092),2.5%CaCl2(Hi=2.150±0.066)and the control(Hi=2.749±0.103)reached statistical significance(P<0.001).Conclusion CaCl2may produce a decrease activity in the hemolysis of candida species.