LC测定水产品中组胺含量

Determination of Histamine in Aquatic Products by LC

高效液相色谱具有检测灵敏度高、定性准确、检出限低、定量分析准确的特点,是目前水产品中生物胺含量分析测定的主要手段。组胺的测定需经衍生,丹酰氯是液相色谱常用柱前衍生试剂,其操作简单、衍生物稳定性好、可定量完成磺酰化反应,有较强的荧光和紫外吸收、灵敏度高、基体干扰不明显等优点,是高效液相色谱检测生物胺中应用最多的柱前衍生试剂。

High performance liquid chromatography has the characteristics of high sensitivity,accurate,low detection limit and accurate quantitative analysis.Determination of histamine by derivatization withdansyl chloride reagent is commonly used in liquid chromatography with pre-column derivatization has theadvantages of simple operation,good stability,can complete the quantitative derivatives sulfonylation,havestrong fluorescence and UV absorption,high sensitivity,matrix interference is not obvious advantages,is thedetection of biogenic amines in HPLC application most of the derivatization reagent.