摘要
本文以马铃薯块茎为研究材料,采用根癌农杆菌介导的瞬时表达方法,通过组织化学染色法检测GUS基因的瞬时表达,并研究了乙酰丁香酮浓度、真空强度、浸染时间和共培养时间对GUS基因瞬时表达的影响。结果表明不同真空强度和共培养时间对GUS基因瞬时表达水平的影响差异极显著,且当真空强度为1 k Pa、共培养时间为2 d时表达水平较高。
The GUS gene was transiently expressed in potato tubers by agrobacterium-mediated transformation and theexpression the GUS was detected by histochemical staining.The effects of acetyligenone concentration,vacuum intensity,immersion time,and total culture time on the expression of GUS gene was investigated.The results show that the differentvacuum strength and co-culture time exhibit the notable influence on GUS expression and the expression levels are higherwhen the vacuum intensity is1kPa and the co-culture time is2days.
作者
舒锐
姚甜甜
李燕
臧传江
焦健
兰成云
刘少军
SHU Rui;YAO Tian-tian;LI Yan;ZANG Chuan-jiang;JIAO Jian;LAN Cheng-yun;LIU Shao-jun(Shandong Light Industry Institute of Agricultural and Sideline Raw Materials,Gaomi 261500,China;College of Horticulture Science and Engineering/Shandong Agricultural University,Tai’an 271018,China)
出处
《山东农业大学学报（自然科学版）》
CSCD
2017年第4期545-548,共4页
Journal of Shandong Agricultural University：Natural Science Edition
关键词
马铃薯
GUS
调控因子
瞬时表达
Potato
GUS
regulatory factors
transient expression
