摘要
该实验以黄果龙葵和龙葵的根尖为实验材料,进行不同的预处理、固定和解离,确定出各种材料适合于核型分析的制片方法。结果表明:龙葵于15℃条件下经0.05%秋水仙素预处理2.5h,固定后用1mol/L HCl酸解后,染色观察,得到的染色体分散,易于染色体计数和形态观察。用此方法对黄果龙葵和龙葵进行核型分析,结果发现:黄果龙葵和龙葵都属于小型染色体,黄果龙葵为四倍体,核型公式为K(4n)=48=4sm+44m,核型不对称系数为56.22%,属于2B核型。龙葵为六倍体,核型公式为K(6n)=72=72m,核型不对称系数为55.89%,属于1B核型。
In this test,the experimental materials are root tips ofSolanum nigrumvar.suaveolensandSolanum nigrum.In order to determine pretreatment methods of the most suitable for karyotype analysis,we optimized processing the root tips by different pretreatment,fixed and different dissociation.Root tips ofSolanum nigrumL.are pretreated with0.05%colchicine for2.5h at15℃.Then root tips were hydrolyzed or6min in1mol/L HCl at60℃.The results showed the chromosome clearer.S.nigrumvar.suaveolensandS.nigrumare both belong to small chromosomes,S.nigrumvar.suaveolensis tetraploid,the karyotype formula is K(4n)=48=4sm+44m,karyotype asymmetry belongs to2B.The karyotype asymmetry coefficient was56.22%.S.nigrumL.is hexaploid,the karyotype formula is K(6n)=72=72m,karyotype asymmetry belongs to1B and the karyotype asymmetry index was55.89%.
作者
徐洪国
祁宏英
顾灵杰
XU Hongguo;QI Hongying;GU Lingjie(College of Life Science and Agroforestry, Qiqihaer University, Qiqihaer, Heilongjiang 161006, China)
出处
《西北植物学报》
CAS
CSCD
北大核心
2017年第2期387-393,共7页
Acta Botanica Boreali-Occidentalia Sinica
基金
齐齐哈尔大学博士科研启动金(340107)
关键词
龙葵
染色体
制片优化
核型
Solanum nigrum L.
chromosome sectioning
chromosome preparation
karyotype