乙型肝炎病毒DNA定量检测与临床的关系

DNA quantifying assay of hepatitis B virus and its clinical significance

目的分析乙型肝炎病毒DNA(HBV-DNA)定量检测与临床的关系。方法随机选取该院乙型肝炎血清标志物阳性患者100例，根据血清学标志物分成4组，同时选取22例健康者为对照组。利用微板核酸杂交技术对5组研究对象进行HBVDNA定量检测，分析检测结果。结果乙肝表面抗原、乙肝E抗原及乙肝核心抗体阳性组的HBV-DNA阳性率较其他组明显升高（P<0.05)。结论HBV复制情况无法借助乙肝血清学标志物进行完全反映，HBV-DNA定量检测能够有效监测HBV感染的病情进展和指导抗HBV治疗。

Objective T To analyze the relationship between quantitative detection of hepatitis B virus DNA(HBV-DNA)and its clinical significance.Methods A total of100patients with hepatitis B serum markers were randomly selected and divided into4groups according to serological markers in the hospital.At the same time,22healthy subjects were chosen as the control group.U-sing microplate hybridization technique,the HBV-DNA quantitative detection was performed on5groups of subjects,and the results were analyzedResults The HBV-DNA positive rate in the hepatitis B surface antigen,hepatitis B E antigen and hepatitis B core an-tibody positive group was significantly higher than that in other groups(P<C〇.05).Conclusion The HBV serological markers can not reflect the replication of HBV,the HBV-DNA quantitative detection can effectively monitor the progress of HBV infection and guide the anti-HBV treatment.