妊娠期PM2.5,暴露影响子代鼠主要脏器发育

PM2.5 exposure during pregnancy causes abnormal development of major organs in offspring mice

目的探讨妊娠期大气细颗粒物PM2.5(颗粒直径≤2.5μm)暴露对子代鼠主要脏器发育的影响。方法孕鼠随机分为空白组、对照组、PM2.5低剂量组、PM2.5中剂量组、PM2.5高剂量组。利用气管滴注方法,建立小鼠妊娠期PM2.5暴露模型。通过HE染色和PAS反应,观察妊娠期暴露于PM2.5后新生子代鼠主要脏器的病理形态学改变,通过电子显微镜观察细胞超微结构变化。结果与空白组和对照组相比,PM2.5模型组新生子代鼠心、肝、脾、肺和肾组织结构均受损,可见炎症等病理改变。子代鼠主要脏器超微结构出现不同程度的细胞核周隙局部增宽、自噬体增多、线粒体嵴模糊和断裂等变化。结论妊娠期暴露于PM2.5可导致新生子代鼠心、肝、脾、肺、肾等主要脏器出现组织形态异常。这可能是PM2.5致生后远期慢性疾病发生的解剖学基础和发育源性病因。

Objective To investigate the effects of atmospheric particulate matter(AD≤2.5μm,PM2.5)exposure on the developmentof major organs in offspring mice during pregnancy.Method Pregnant mice were randomly divided into blank,control,PM2.5low,medium and high dose groups.Rapid mice trachea drip improved method was used to establish the pregnant mice PM2.5exposured model.The pathological changes of main organs in neonatal mice exposed to PM2.5during pregnancy were evaluated byHE staining and PAS reaction,while the cellular ultrastructural changes were analyzed by transmission electron microscopy.ResultsCompared to the blank and control groups,the tissue organization of major organs including heart,liver,spleen,lung and kidney in neonatalmice of PM2.5group were abnormal with obvious inflammatory infiltration.Changes at ultrastructural level were also observedin PM2.5group,including widened perinuclear gap,increased autophagosomes,and mitochondrial cristae fragmentation.ConclusionPM2.5exposure during pregnancy results in abnormal tissue organization and morphology of major organs in neonatal mice,whichmay potentially be the anatomical and pathological root cause of chronic diseases in later life.