固相碳源脱氮同步去除壬基酚的试验研究

Simultaneous removal of nitrogen and nonylphenol using solid phase carbon source

采用一种基于PHBV研发的新型固相碳源NS,构建同步硝化反硝化脱氮生物反应器,同时探究常见的内分泌干扰物壬基酚(NP)在系统中的同步去除特性,并运用Illumina Mi Seq高通量测序和荧光定量PCR(q PCR)技术,对微生物群落进行解析.反应器运行效果良好,硝酸盐氮和氨氮去除率可分别达到96.18%和82.54%,出水COD平均浓度为27.48mg/L,壬基酚平均去除率为81.17%.高通量测序结果表明,Dechloromonas(脱氯单胞菌属)和Zoogloea(动胶菌属)等报道的具脱氮作用的菌属在无壬基酚条件下占据优势地位.加入壬基酚的条件下,菌种总数和α多样性降低.荧光定量PCR结果表明,系统中氮循环基因丰度较高,nir S基因丰度最大,而amo A基因丰度最低.进水中存在壬基酚的条件下碳源表面nir S基因丰度较低.

The simultaneous nitrification and denitrification bioreactors in this research were packed with a new type of solid phase carbon source NS,which was invented based on PHBV.The removal rate of nitrogen and nonylphenol(NP)in the reactor was investigated.Illumina MiSeq high-throughput sequencing and quantitative PCR(qCPR)were applied to analyze the microbial community structure and functional genes.The removal rates of nitrate and ammonia nitrogen were96.18%and82.54%,respectively.Average COD concentration was27.48mg/L in effluent.The average removal efficiency of NP was81.17%.The results of high-throughput sequencing illustrated that the previously reported denitrifiers including Dechloromonas,Rhodocyclaceae and Zoogloea were the dominant microbe genus on the surface of solid carbon when NP was not added.The estimated total number of bacteria species andαdiversity decreased after NP was added in the influent.According to outcomes of qPCR,functional genes of nitrogen cycle had rich abundance in the bioreactor.Abundances of nirS gene and amoA gene were the highest and lowest,respectively.However,nirS abundance reduced when NP was added in the influent.