红光响应溶菌酶PLGA纳米囊的制备与表征

Preparation and Characterization of PLGA Nanocapsules Containing Red Light Responsive Lysozyme

以聚乳酸-羟基乙酸共聚物(PLGA)为囊材,添加脱镁叶绿酸,采用复乳法制备红光响应溶菌酶纳米囊。以包封率、载药量和纳米囊粒径为指标优化配方,对其结构、热流变性和光控释测定。获得优化的PLGA、溶菌酶及脱镁叶绿酸用量配比为55:10:1,纳米囊包封率为81.3%±4.5%,载药量为13.1%±1.6%,平均粒径为(667±35)nm,电位(-39.6±4.6)m V。纳米囊呈紧密球形,光敏剂分布在囊壳,650 nm光照30 min药物释放明显加快。此新型纳米囊在不光照时具有增强药物稳定和缓释作用,而红光可促进释药,因而可实现光控靶向。

Poly(lactic-co-glycolic acid)(PLGA)was used as an encapsulating material together withpheophorbide to prepare red light responsive lysozyme nanocapsules by a double emulsion method.Encapsulation efficiency,drug loading and particle size were used as main parameters in formulationoptimization,and nanocapsule structure,thermo rheological property and photo responsiveness were analyzed.The optimal formulation is PLGA:lysozyme:pheophorbide=55:10:1.Nanocapsules with encapsulationefficiency of81.3%±4.5%,drug loading of13.1%±1.6%,average particle size of(667±35)nm and zetapotential of(-39.6±4.6)mV are obtained and the nanocapsules have compact spherical shape withpheophorbide distributed in outer layer.Drug release is accelerated under illumination at650nm for30min.This new nanocapsule can keep drug stable and sustainable release without illumination,and drug can bereleased quickly under red light,which made red light controlled release achievable.