不同蜜炙方法对红芪中毛蕊异黄酮和芒柄花素含量的影响

Effects of Different Honey Sunburn Methods on Calycosin and Formononetin in Hedysari Radix

目的比较不同蜜炙方法对红芪中毛蕊异黄酮和芒柄花素含量的影响,为其蜜炙最佳工艺的建立提供依据。方法采用传统炒制、烘制、微波制的方法蜜炙红芪;采用Agilent HC-C18色谱柱(4.6 mm×250 mm,5μm);流动相为乙腈-0.01%磷酸水溶液,梯度洗脱(0~12 min,30%~33%乙腈;12~13 min,31%~40%乙腈,13~25 min,40%乙腈),流速1.0 mL/min,检测波长248 nm,柱温30℃,进样量10μL。结果红芪不同蜜炙品中毛蕊异黄酮和芒柄花素含量存在差异。红芪烘制炙品中毛蕊异黄酮和芒柄花素含量最高,分别为7.911 6、49.699 6μg/g;红芪传统炙品中二者含量最低,分别为4.776 7、37.291 0μg/g;红芪生品和红芪微波炙品中二者含量相当,分别为5.080 2、42.798 9μg/g和3.983 9、42.314 5μg/g。结论不同蜜炙方法对红芪中毛蕊异黄酮和芒柄花素含量有一定影响,烘制炙法是红芪蜜炙的最优方法。

Objective To compare the effects of different methods of honey sunburn on the contents of calycosin and formononetin in Hedysari Radix;To provide the basis for the establishment of the optimum processing technology.Methods By frying(traditional method),baking,and microwave methods put Hedysari Radix under honey sunburn.Agilent HC-C18column(4.6mm×250mm,5μm)was used;the mobile phase was acetonitrile-0.01%phosphoric acid solution,gradient elution(0-12min,30%-33%acetonitrile;12-13min,31%-40%acetonitrile;13-25min,40%acetonitrile)with velocity of1.0mL/min;detection wavelength was248nm;the column temperature was30℃;sample volume was10μL.Results There was statistical significance in the contents of calycosin and formononetin of different methods of honey sunburn for Hedysari Radix.Among them,the contents of calycosin and formononetin in Hedysari Radix processed by honey roast were the highest,7.9116and49.6996μg/g,respectively;the contents of calycosin and formononetin in Hedysari Radix processed by traditional method were the lowest,4.7767and37.2910μg/g,respectively;the contents of calycosin and formononetin in raw Hedysari Radix and Hedysari Radix by honey microwave method were the same,5.0802,42.7989μg/g,and3.9839,42.3145μg/g,respectively.Conclusion Different honey sunburn methods for the contents of calycosin and formononetin in Hedysari Radix have certain effects,and honey roast method is the optimum method.