星点设计-效应面法优化大花八角莽草酸提取工艺

Optimization of Extraction Process for Shikimic Acid from Illicium Macranthum A. C. Smith with Central Composite Design and Response Surface Method

目的探索获取回流提取大花八角中莽草酸的最佳工艺条件。方法以莽草酸得率为指标,采用效应面法(response surface methodology,RSM)中的星点设计(central composite design,CCD)对大花八角中莽草酸的回流提取工艺进行优化,建立各因素与响应值之间的数学模型。结果确定最佳提取工艺条件为在甲醇溶剂分数25%、液料比15∶1(mL/g)的条件下,浸泡12 h,在温度70℃条件下,提取1次,每次1.5 h。该条件下大花八角莽草酸提取量为92.546 6 mg/g,提取率实测值与预测值偏差小于1%。结论星点设计-效应面法优选大花八角中莽草酸的提取工艺,方法简单,可靠,预测性好。

Objective To study the optimal extraction conditions of shikimic acid from Illicium macranthum A.C.Smith.Methods With the yield of shikimic acid as index,the central composite design(CCD)experiment and the response surface methodology(RSM)were conducted to build a model between the factors and response value to optimize reflux extraction process.Results The optimum conditions were methanol concentration25%,liquid-to-materia15∶1(mL/g),soaking for12h,temperature70℃,extracting1time,each time1.5h.Under these conditions,the extraction capacity of shikimic acid was92.5466mg/g,bias between observed value and predicted values was less than1%.Conclusion Optimum extraction process for shikimic acid from Illicium Macranthum with central composite design and response surface method is simple,reliable and highly predictive.