肺宁颗粒对大鼠放射性肺炎及细胞因子表达的影响

Efficacy of the Feining capsules on radiation pneumonitis and expression of cytokines in rats

目的:为临床应用肺宁颗粒防治放射性肺炎提供实验依据。方法:SPF级SD雄性大鼠32只,随机分为正常对照组、模型组、肺宁颗粒组和地塞米松组,每组8只。利用6mv X线,对全胸进行单次照射,总剂量为20Gy,建立放射性肺炎大鼠模型。除正常对照组不进行胸部放射照射外,其余三组均进行胸部放射照射。肺宁颗粒组进行灌胃输入药液0.05g/100g体质量,地塞米松组进行灌胃输入药液0.05mg/100g体质量,正常对照组和模型组给予同体积生理盐水灌胃。照射后第1天开始用药,每日1次,连续灌胃至照射后满6周处死动物。观察各组大鼠肺大体改变并计算肺系数;HE染色观察肺组织病理变化;ELISA法检测血清白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、转化生长因子-β_1(TGF-β_1)水平。结果:肺宁颗粒组及地塞米松组肺系数,肺组织炎性及纤维化改变,血清IL-6、TNF-α、TGF-β_1水平均显著低于模型组(均P<0.05),且上述各项指标肺宁颗粒组均优于地塞米松组(均P<0.05)。结论:肺宁颗粒可能通过抑制血清中IL-6、TNF-α、TGF-β_1的合成从而对大鼠放射性肺炎起到防治作用。

Objective:To observe efficacy of the Feining capsules on radiation pneumonitis plus expression of cytokines in rats.Methods:32SD male rats of SPF grade were randomly divided into the normal control group,model group,the Feining capsules group and dexamethasone group,8rats in each group.The whole chest was given a single exposure by the6mv X line,the total dose of20Gy,and radioactive pneumonia rat model was established.Excepted for the normal control group,the other groups were given chest radiotherapy.The Feining capsules group was treated with the Feining capsules;the dexamethasone group took dexamethasone;the normal control group and the model group received normal saline.Results:Lung coefficient,inflammation of the lung tissue and fibrosis,the levels of IL-6,TNF-αand TGF-β1in the Feining capsules group and dexamethasone group were significantly lower than those in the model group(P<0.05);and the above indexes in the Feining capsules group were better than those in the dexamethasone group(P<0.05).Conclusion:The Feining capsules can inhibit the formation of IL-6,TNF-αand TGF-β1in serum and thus play a role in the prevention and treatment of radioactive pneumonia in rats.