体外模拟消化对车前子来源毛蕊花苷抑制黄嘌呤氧化酶活性的影响

Effects of in Vitro Simulated Degradation on the Xanthine Oxidase Inhibitory Activity of Verbascoside from Plantago asiatica L.

研究体外模拟消化各阶段的条件对车前子来源毛蕊花苷抑制黄嘌呤氧化酶活性的影响。结果发现毛蕊花苷含量在模拟消化作用4 h后降低,降低程度最高的是肠菌孵育样液,毛蕊花苷含量减少率为(71.26±0.96)%,其次是累加消化样液,达(28.46±0.50)%。模拟消化后各样液的黄嘌呤氧化酶抑制作用均有所增加,增加程度最大的为肠菌孵育样液(P<0.05),其次为累加消化样液。经高效液相色谱-质谱联用分析发现,各消化样液中均能检出毛蕊花苷的代谢苷元咖啡酸和羟基酪醇,活性实验表明后两者对黄嘌呤氧化酶的体外抑制活性高于前体毛蕊花苷。毛蕊花苷降解成具有黄嘌呤氧化酶体外抑制活性的产物可能是其在体内表现生理活性的途径之一。

In this study,the effects of different stages of in vitro simulated digestion on the stability of Plantago asiatica L.seed verbascoside(PSV)were explored.It was found that the concentration of PSV in all simulated digestion systems was reduced after digestion for4h,and the highest reduction of(71.26±0.96)%was observed in the gut bacterial culture,followed by the simulated gastrointestinal digestion system((28.46±0.50)%).After simulated digestion,xanthine oxidase inhibitory activity in various simulated digestion systems increased,in particular in the gut bacterial culture(P<0.05),followed by the simulated gastrointestinal digestion system.The aglycone metabolites of verbascoside,caffeic acid and hydroxytyrosol,were detected from all simulated digestion systems by LC-MS.The in vitro xanthine oxidase inhibitory activity of caffeic acid and hydroxytyrosol was higher than that of verbascoside.The degradation of verbascoside into xanthine oxidase inhibitory metabolites is likely to be one of the effective ways to exert its physiological activity in vivo.