高压微射流处理对葡萄籽原花青素组分构成的影响

Effect of Dynamic High-Pressure Microfluidization on the Composition of Grape Seed Proanthocyanidins

研究高压微射流处理对从葡萄籽中提取得到的原花青素中儿茶素、原花青素B2和表儿茶素含量的影响。将原花青素在不同的处理压力(100、140、180、220 MPa和260 MPa)条件下进行1~4次循环处理,采用高效液相色谱进行定量分析。结果表明:随着处理压力的增加,儿茶素、原花青素B2和表儿茶素含量随之增加;随着处理次数的增加,含量基本呈先增加后减少的趋势;与其他处理条件下的原花青素含量相比,260 MPa条件下循环处理两次,儿茶素(0.163 2 mg/m L)、表儿茶素的质量浓度(0.128 6 mg/m L)最高;260 MPa条件下循环3次获得的原花青素B2质量浓度(0.057 6 mg/m L)最高。说明高压微射流处理会破坏高聚原花青素分子链之间的非共价键,使其解聚生成活性更高的单体、低聚体。

In this study,the effect of dynamic high-pressure microfluidization(DHPM)on the contents of catechin,procynidin B2and epicatechin in grape seed proanthocyanidin extract(GSPE)was studied.The proanthocyanidins were subjected to different cycles(1-4)of DHPM at different pressures(100,140,180,220and260MPa)and then analyzed by high performance liquid chromatography(HPLC).The results showed that the contents of these substances increased with increasing DHPM pressures,but they rose firstly and then declined with increasing number of DHPM cycles.Two DHPM cycles at260MPa resulted in the highest contents of catechin and epicatechin(0.1632and0.1286mg/mL,respectively)among all treatments and the highest content of procynidin B2of0.0576mg/mL was obtained after three DHPM cycles.Hence,it was demonstrated that DHPM could destroy the non-covalent bonds between polymeric proanthocyanidin chains and consequently depolymerize them into oligomers and monomers with higher biological activity.