摘要
为明确山东地区番茄褪绿病毒(Tomato chlorosis virus,To CV)侵染茄子的情况,采用RT-PCR分子检测鉴定疑似感染To CV的茄子叶片样品及发病茄子植株上的烟粉虱(Bemisia tabaci)体内To CV的带毒情况。结果表明:10份茄子叶片样品中有6份扩增得到约463 bp的特异条带,经测序与Gen Bank中To CV序列相似性达到99.0%以上,To CV检出率为60%;系统进化分析显示,山东寿光地区To CV茄子分离物cp-1与北京番茄分离物BJ亲缘关系最近。温室内发病茄子植株上的烟粉虱体内携带To CV,带毒率为70%。
In order to clarify the occurrence of Tomato chlorosis virus(ToCV)on eggplant in ShandongProvince,this paper detected and identified by RT-PCR the samples of eggplant leaves prospectively infectedby ToCV and also the viruliferous percentage of ToCV in Bemisia tabaci adults on infected eggplant plants.Theresults showed that463bp specific fragments were amplified from the detected samples and6samples were positivefor ToCV and the positive percentage was60%.The CP gene of positive samples were cloned and sequenced,sharing over99.0%nucleotide identity with a tomato isolate from Beijing(BJ).Moreover,ToCV could bedetected in B.tabaci on ToCV-infected eggplant plants and the viruliferous percentage of ToCV was70%.
作者
刘永光
代惠洁
杨园园
陈鹏
竺晓平
赵静
LIU Yong-guang;DAI Hui-jie;YANG Yuan-yuan;CHEN Peng;ZHU Xiao-ping;ZHAO Jing(Weifang College of Science & Technology,Key Laboratory of Protected Horticulture for Universities in Shandong Province,Shouguang 262700,Shandong,China;College of Plant Protection,Shandong Agricultural University,Key Laboratory for Biology of Vegetable Diseases and Insect Pests in Shandong Province,Tai’an 271018,Shandong,China)
出处
《中国蔬菜》
北大核心
2017年第9期41-46,共6页
China Vegetables
基金
山东省自然科学基金项目(ZR2015CQ022)
潍坊市科技发展计划项目(2016ZJ1113)
山东省农业重大应用技术创新课题
关键词
山东地区
茄子
番茄褪绿病毒
分子检测
烟粉虱
传毒
Regions in Shandong Province
Eggplant
Tomato chlorosis virus(ToCV)
Molecular identification
Bemisia tabaci
Viral transmission
