PCR技术在使用亚胺培南治疗的患者中检测真菌感染的临床意义

Clinical application of PCR technology for detection of fungus in patients treated with imipenem

目的:探讨荧光PCR技术在使用亚胺培南治疗的患者中检测真菌感染的临床意义。方法:选择31例使用亚胺培南治疗的患者,收集治疗前后的标本各2份。1份采用传统培养法,1份采用PCR法检测真菌。结果:两种方法均检测出白色念珠菌、热带假丝酵母菌、光滑假丝酵母菌。PCR法检出阳性11例,阳性率35.48%;传统培养法检测阳性5例,阳性率16.12%。5 d PCR检测阳性率5.2%,7 d检出阳性率35.48%。结论:PCR检测真菌感染的稳定性好,敏感性和特异性均较理想。

Objective:To explore the clinical significance of PCR technology for detection of mycotic infection in patients treated with imipenem.Methods:31patients treated with imipenem were selected.We collected two specimens before and after treatment.One was given conventional culture to detect fungi,and one was given PCR to test fungi.Results:Candida albicans,candida tropicalis and candida tropicalis were detected by two methods.In the PCR method,11cases were positive,and the positive rate was35.48%.In the traditional culture method,5cases were positive,and the positive rate was16.12%.At5days,the positive rate of PCR detection was5.2%.At7days,the positive rate of PCR detection was35.48%.Conclusion:The stability of PCR for detecting fungal infections was good,and the sensitivity and specificity were ideal.