陆地棉SRO基因家族的鉴定及表达分析

Genome-wide Identification and Expression Analysis of SRO Genes Family in Gossypium hirsutum L.

SRO是植物特有的类RCD-ONE蛋白家族,在植物抵御各种生物和非生物胁迫过程中具有重要的作用。本研究利用生物信息学方法从陆地棉遗传标准系TM-1(Gossypium hirsutum L.acc.TM-1)基因组中鉴定到12个SRO基因家族成员。多重序列比对及进化树分析表明,12个陆地棉SRO均含有PARP和RST结构域,聚为3类,即A、B和C亚家族。转录组数据分析表明,TM-1全基因组中的Gh_D12G1442在茎中优势表达,Gh_D05G2064在萼片和雄蕊中优势表达;Gh_A05G3788在雄蕊中优势表达,Gh_A12G1318在雄蕊和纤维中优势表达;Gh_A12G2480、Gh_D12G2608、Gh_A05G2257和Gh_D05G2516在纤维中优势表达;Gh_A08G1390、Gh_D08G1685、Gh_A12G2663和Gh_D12G2054具有较为广泛的组织表达。逆境胁迫处理试验表明,Gh_A08G1390明显受冷处理和盐处理的诱导,Gh_D12G2054、Gh_D08G1685、Gh_A12G2663基因在不同的胁迫处理下受到不同程度的诱导。

RCD-ONE proteins family(SRO)is a kind of plant-specific proteins,which plays an important role in plants in response to different abiotic stress.In this study,12SRO genes were preliminary identified in Gossypium hirsutum L.standard line TM-1by bioinformatics analysis.Multiple sequence alignment and phylogenetic tree analysis showed that these SROs contain PARP and RST domains,and could be divided into A,B,and C subfamilies.Transcriptome data analysis showed that in the whole genome of TM-1,Gh_D12G1442advantageously expressed in the stem,Gh_D05G2064in the sepals and stamens,Gh_A05G3788advantage expression in stamens,Gh_A12G1318in stamens and fiber,Gh_A12G2480,Gh_D12G2608,Gh_A05G2257,Gh_D05G2516advantage expression in fiber;Gh_A08G1390,Gh_D08G1685,Gh_A12G2663,and Gh_D12G2054in wider organs.In stress treatment showed that Gh_A08G1390was obviously induced by cold and salt treatments,Gh_D12G2054,Gh_D08G1685,Gh_A12G2663gene were induced try different stresses to a different degrees.