CK7,CEA,CA125,KI67基因在卵巢肿瘤手术盆腔冲洗液沉渣中的诊断与研究

The Diagnosis and Research of CK7,CEA,CA125 and KI67 Genes Combined with Sediment Immersion in Pelvic Rinses in Ovarian Tumor

目的探讨盆腔冲洗液沉渣切片联合免疫组化检测CK7、CEA、CA125、Ki-67在卵巢肿瘤诊断中的临床意义。方法收集2014年1月~2016年12月我院卵巢肿瘤患者手术中的盆腔冲洗液72例,进行盆腔冲洗液沉渣包埋,沉渣包埋后切片行HE染色和CK7、CEA、CA125、Ki-67的免疫组织化学染色。结果沉渣包埋切片HE染色观察,上皮性恶性肿瘤检出率为44.4%(32/72)。以沉渣包埋组织HE切片诊断检出结果为标准进行分组,分为上皮性卵巢恶性肿瘤组(32例)和非恶性肿瘤组(40例),计算各组免疫组化标记物的表达情况。卡方检验显示,各组间CK7、CA125和ki-67的阳性表达率具有显著性差异(P<0.01),CEA表达无显著差异(P>0.05)。结论盆腔冲洗液沉渣包埋组织石蜡切片联合免疫组化检测CK7、CEA、CA125、Ki-67可以协助诊断卵巢恶性肿瘤,并为明确恶性肿瘤细胞组织来源提供了客观依据。

Objective To investigate the diagnostic value of sediment immersion in pelvic rinsesand immunhistochemical detection of CK7、CEA、CA125and Ki67in ovarian tumor.Methods72cases of sediment immersion in pelvic rinses of ovarian tumor were collected,embededed,siliced forimmunohistochemical detection of CK7,CEA,CA125and Ki67.Results The positive expression ofCK7,CA125and ki-67in ovarian malignant tumor was higher than that in ovarian tumor(P<0.01),butunrelated with CEA(P>0.05).Conclusion Sediment immersion in pelvic rinses and immunhistochemical detection of CK7、CEA、CA125and Ki67in ovarian tumor can help to diagnosis the ovarian malignant tumor.