ADAM33基因T2位点多态性与支气管哮喘的关系性分析

Relationship between T2locus polymorphism of ADAM33gene and bronchial asthma

目的探索整合素-金属蛋白酶33（A Disintegrin And Metalloproteinase33，ADAM33）基因T2位点多态性与支气管哮喘的相关性。方法研究对象选取本院呼吸内科2015年6月～2016年12月期间收治的哮喘患儿120例作为观察组，同期入本院体检的120名健康儿童作为对照组。患儿入院均行上肢肘静脉抽血并提取DNA，随后进行聚合酶链反应-限制性片段长度多态性分析（Polymerase Chain Reaction-Restriction Fragment Length Polymorphism，PCR-RFLP）和测序分型。比较2组患儿按照Hardy-Weinberg平衡定律检验结果、T2位点基因型分布频率以及T2位点基因频率分布及其与哮喘相对危险度。结果2组受试对象T2位点基因频率均满足Hardy-Weinberg平衡定律，具有良好的群体代表性；观察组受试对象T2等位基因中A的基因频率明显高于对照组，2组受试对象比较差异有统计学意义(χ2=8.09，P=0.00)，且A等位基因的增加是患哮喘的高危因素(OR=2.32)；观察组受试对象T2位点AG基因型频率明显高于对照组，2组受试对象间比较差异有统计学意义(χ2=4.21，P=0.04)，且AG等位基因的增加是患哮喘的高危因素(OR=1.89)。结论ADAM33基因T2位点多态性与支气管哮喘明显相关，其能显著增加患支气管哮喘的可能性。

Objective To explore the relationship between the polymorphism of integrin-metalloproteinase-33(ADAM33)gene and bronchialasthma.Methods Subjects were selected from the Department of Respiratory Medicine in our department from June2015to December2016in thetreatment of120cases of asthma patients as the observation group,the same period into our hospital physical examination of120healthy children asthe control group.The patients were enrolled in the upper extremity elbow vein and extracted DNA,followed by polymerase chain reaction-restrictionfragment length polymorphism(PCR-RFLP)and sequencing.The frequency of T2genotype distribution and the frequency distribution of T2locusand the relative risk of asthma were compared according to Hardy-Weinberg equilibrium law.Results The frequency of A gene in the T2allele of thesubject was significantly higher than that of the control group.The frequency of A gene in the T2allele was significantly higher than that in the controlgroup(P<0.05)There was significant difference between the two groups(χ2=8.09,P=0.00),and the increase of A allele was the risk factor of asthma(OR=2.32);the observation group T2gene AG(χ2=4.21,P=0.04),and the increase of AG allele was the risk factor of asthma.The frequency ofasthma was significantly higher than that of control group(OR=1.89).Conclusion ADAM33gene T2locus polymorphism is significantly associatedwith bronchial asthma,which can significantly increase the risk of bronchial asthma.