摘要
为探讨Genomic-SSR与EST-SSR标记在桉树种间的遗传差异,本研究利用两种不同来源的SSR标记对6种桉树进行遗传差异性分析。分析结果表明:Genomic-SSR标记在6种桉树中具有高的遗传多样性,EST-SSR标记在揭示不同基因型间遗传关系方面更具优势。对两种标记进行聚类分析发现,EST-SSR标记比Genomic-SSR标记能更准确地鉴别基因型。两种不同来源SSR标记的遗传差异性,为更有效利用SSR标记在桉树遗传多样性分析、种质鉴定等相关研究提供了理论支持。
To explore the genetic differences between genomic-SSR and EST-SSR markers in different species of Eucalyptus,the genetic diversity of6species were analyzed using two types of SSR markers in the study reported here.Genomic-SSRs were found to be more polymorphic than EST-SSRs in all6Eucalyptus species,with EST-SSRsproving to be more powerful for revealing genetic relationships among different genotypes than genomic-SSRs.Moreover,cluster analyses indicated that EST-SSR markers could identify different genotypes more accurately.The genetic differences revealed in this study for genomic-SSR and EST-SSR markers will provide theoretical support for rational future use of SSR markers in studies of Eucalyptus genetic diversity,germplasm identification and other related research.
作者
刘果
谢耀坚
陈鸿鹏
吴志华
LIU Guo;XIE Yao-jian;CHEN Hong-peng;WU Zhi-hua(China Eucalypt Research Centre, Zhanjiang 524022,Guangdong,China)
出处
《桉树科技》
2017年第3期1-8,共8页
Eucalypt Science & Technology
基金
中央级公益性科研院所基本科研业务费专项资金(CAFYBB2016MB004)
