纳米金–壳聚糖修饰电极循环伏安法测定抗坏血酸

Determination of Ascorbic Acid by Cyclic with Electrodes Modified by Au Nanoparticles and Chitosan

介绍纳米金–壳聚糖修饰电极的制备方法及其测定抗坏血酸的分析应用。采用电沉积方法,将氯金酸与壳聚糖的混合电解液直接共沉积,制备了壳聚糖–纳米金修饰玻碳电极的电化学传感器。利用循环伏安法研究了抗坏血酸浓度、p H值等对抗坏血酸在修饰电极上的电化学行为的影响。实验结果表明,修饰电极对抗坏血酸具有良好的电催化氧化作用,抗坏血酸浓度在5×10^(–5)~1×10^(–3) mol/L范围内线性良好,回归方程为I_p=0.433 8c+0.881 9,相关系数为0.998 71。该法可指导纳米金–壳聚糖修饰电极的制备及抗坏血酸含量的测定。

It was introduced of the method to prepare the electrodes modified by Au nanoparticles and chitosan and the usage to determine ascorbic acid.The electrochemical sensor of glassy carbon electrode modified by chitosan and gold nanoparticles were prepared by electrodeposition with chloroauric acid and chitosan as the mixed electrolyte.The effects of ascorbic acid concentration and pH value on the electrochemical behavior of ascorbic acid over the modified electrodes were investigated by cyclic voltammetry.The results showed that the ascorbic acid possessed high activity for electric catalytic oxidation on the modified electrodes.A good linear relationship was shown when the concentration of ascorbic acid changed from5×10-5mol/L to1×10-3mol/L.The equation of linear regression was Ip=0.4338c+0.8819with the linear correlation coefficient of0.99871.This method can guide to the preparation of the electodes modified by Au nanoparticales and chitosan and determination of ascorbic acid.