拟南芥RINGE3连接酶AtASSR1在盐胁迫响应中的功能初探

Preliminary Study on Function of Arabidopsis RING E3 Ligase AtASSR1 in Salt Stress Response

【目的】构建拟南芥AtASSR1转基因株系,研究并初步揭示RING E3连接酶AtASSR1在盐胁迫应答中的作用。【方法】利用农杆菌介导的花序浸染法,成功获得AtASSR1基因插入的纯合转基因株系,并通过检测盐胁迫处理前后,转基因株系及野生型、atassr1突变体植株中生理生化指标来初步揭示AtASSR1的功能。【结果】通过检测盐胁迫处理前后,不同基因型株系的H_2O_2、过氧化氢酶活性、丙二醛的含量,发现转基因株系中的过氧化氢酶活性最低,而积累的H_2O_2与丙二醛含量最多;分析脯氨酸含量发现,盐胁迫处理后突变体株系的脯氨酸含量积累最多。【结论】E3连接酶AtASSR1可能在介导植物响应盐胁迫等非生物胁迫的应答中发挥重要生理功能。

Objective】The AtASSR1transgenic plants were constructed and studied to investigate thefunction of RING E3ligase AtASSR1plays an important effect on the responses of Arabidopsis to saltstress.【Method】AtASSR1homozygous transgenic lines were successfully constructed through the Agrobac原terium tumefaciens-mediated transformation.This studies was performed to preliminarily reveal thefunction of AtASSR1by analyzing the physiological and biochemical indexes in wild-type,atassr1mu原tant,and transgenic lines treated with or without NaCl.【Results】The results show that the transgenic lineshave the lowest activity of CAT and the highest content of H2O2and MDA by analyzing the content ofH2O2,catalase(CAT),and Malondialdehyde(MDA)in different genotypes of lines treated with or with原out NaCl.The atassr1mutant accumulates the highest content of proline after salt stress by analyzing thecontent of proline in different genotype lines.【Conclusion】All of theseresultssuggested that AtASSR1may play an important physiological function in plant abiotic stress responses.