巴莲莲子生物碱提取物对人肝癌细胞HepG2的体外抗癌效果

In Vitro Anticancer Activity of Alkaloids Extracted from Ba Lotus Seeds against Human Hepatoma HepG2 Cells

本研究对巴莲莲子生物碱提取物的体外抗癌效果进行研究,以证实巴莲莲子生物碱提取物的生理活性作用。采用噻唑蓝法、4’,6-二脒基-2-苯基吲哚染色法和实时荧光定量聚合酶链式反应法检测巴莲莲子生物碱对人肝癌细胞HepG2的体外增殖抑制作用和凋亡诱导作用。巴莲莲子生物碱可以抑制HepG2细胞的体外增殖,但是对正常肝细胞L02无毒性,不影响其增殖。通过对癌细胞形态的观察发现随着巴莲莲子生物碱质量浓度的提高,更多的癌细胞被诱导凋亡从而死亡。同时,巴莲莲子生物碱能上调HepG2细胞的Bax、caspase-3、caspase-8、caspase-9、p53、p21基因表达和下调Bcl-2、Bcl-x L基因表达,从而促进癌细胞凋亡。巴莲莲子生物碱是一类生物活性成分,实验条件下具有较好的体外抗癌效果。

In this study,the in vitro anticancer activity of alkaloids extracted from Ba lotus seeds was determined to confirm its physiological activity.3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide(MTT),4',6-diamidino-2-phenylindole(DAPI)staining and quantitative real-time polymerase chain reaction(qRT-PCR)methods were used to determine the potential of the alkaloids for inhibiting the proliferation and inducing the apoptosis of human hepatoma HepG2cells in vitro.The alkaloids from Ba lotus seeds could inhibit the proliferation of HepG2cells,but had no toxicity to normal liver cells L02.From morphological observation,it was shown that the alkaloids at higher concentrations could induce more significant apoptosis in cancer cells and lead to cell death.Meanwhile,the alkaloids could up-regulate the mRNA expression levels of Bax,caspase-3,caspase-8,caspase-9,p53and p21,and down-regulate the mRNA expression levels of Bcl-2and Bcl-xL in HepG2cells,thereby promoting apoptosis in cancer cells.Therefore,the alkaloids from Ba lotus seeds are a class of bioactive components with excellent anticancer effect in vitro.