表没食子儿茶素没食子酸酯对乳牙早期釉质龋的再矿化作用

Study on application of natural medicine epigallocatechin gallate for primary enamel caries remineralization

目的通过体外实验研究,观察天然药物表没食子儿茶素没食子酸酯对乳牙早期釉质龋的再矿化作用。方法将门诊因乳牙滞留拔除的上颌乳中切牙30颗按照随机数字表分为3组,实验组(表没食子儿茶素没食子酸酯组)、阳性对照组(Na F组)和空白对照组(人工唾液组),每组10颗,进行体外实验。采用显微硬度计测定标本脱矿前后和p H循环后的硬度值,扫描电镜观察乳牙釉质块再矿化后表面形态结构的改变。结果 p H循环后3组乳牙釉质块表面显微硬度组间两两比较差异均具有统计学意义(F=1 199.975,P<0.05),阳性对照组和实验组较空白对照组乳牙釉质龋的表面显微硬度高(q=41.986,P<0.05 vs实验组;q=68.174,P<0.05 vs阳性对照组),但表没食子儿茶素没食子酸酯再矿化效果较Na F低(q=26.188,P<0.05)。扫描电镜的结果显示,实验组和阳性对照组在乳牙釉质表面均有大量沉积物存在,空白对照组的乳牙釉质表面呈蜂窝状,凹凸不平,沉积物较少。结论表没食子儿茶素没食子酸酯在体外实验的研究中,具有促进乳牙早期人工釉质龋再矿化的作用。

Objective This in vitro study aimed to investigate the remineralization effect of the natural medicineepigallocatechin gallate on artificial enamel caries in primary human teeth.Methods We divided30sound primary upper anterior teeth into3groups according to a random number table,including experimental group(epigallocatechin gallate group),positive control group(NaF group)and blank control group(artificial saliva group),with10teeth in each group.After test in vitro,Micro Hardness Tester was applied to measure hardness of samples before and after demineralization.Scanning electron microscopy(SEM)was used to observe the result of primary enamel surface remineralization.Results A significant increase in enamel surface microhardness between the three groups after remineralization(F=1199.975,P<0.05).The difference between2groups was compared with each other among3groups.Statistical significance was found(vs experimental group q=41.986,P<0.05;vs positive control group q=68.174,P<0.05),suggesting that both positive control group and experimental group could promote the remineralization of primary enamel,and the effect of epigallocatechin gallate was weaker than NaF(q=26.188,P<0.05).The results from SEM indicated that there was large amount of sediment on the surface of primary enamel surface of incisors in the experimental group and positive control group,while primary enamel surface of incisors in the blank control group was honeycomb and uneven,with less sediment.Conclusion Based on this in vitro study,epigallocatechin gallate can promote the remineralization of demineralized enamel of primary teeth,indicating its potential use as a natural remineralization medicine.