短期快速失血对机体脂质过氧化水平影响的实验研究

Experimental Study on the Change of Organism Lipid Peroxidation Level in Short Term Rapid Blood loss

目的探讨短期快速失血对机体脂质过氧化水平的影响。方法选择普通级新西兰兔11只,通过急性失血方式建立收缩压下降曲线,形成快速失血模型,测定收缩压基础值(T1),当收缩压降至基础值2/3(T2)、基础值1/2(T3)、基础值1/3(T4)时采集血样,采用酶联免疫吸附分析检测各位置血清丙二醛(malondialdehyde MDA)、过氧化氢酶(hydrogen peroxide enzyme CAT)、谷胱甘肽过氧化物酶(glutathione oxidation reductase GSH-PX)活性,并予以统计分析。结果 MDA:B组低于C组、C组低于D组,各时间水平间差异无统计学意义(P>0.05);CAT:A组高于B组、B组高于C组、C组高于D组,T1-T2、T2-T3、T3-T4各组间差异无统计学意义(P>0.05),其余组间差异存在统计学意义(P<0.05);GSH-Px:A组高于B组、B组高于C组、C组高于D组,T1、T2间差异无统计学意义(P>0.05),其余组间差异存在统计学意义(P<0.05)。结论短期快速失血对机体抗氧化酶CAT、GSH-Px具有明显影响,可致CAT、GSH-Px活性下降,而反映脂质过氧化程度的MDA水平则上升,失血量与CAT、GSH-Px指标间相关性具有统计学意义。

Objective Study on the changes of organism lipid peroxidation level in short term rapid blood loss.Methods The rapid blood loss model of the gradient decerease in the systolic blood pressure was eatablished in11new zealand rabbits on ordinary level.The arterial blood during the different phase of shock,i.e.when the systolic pressures we r e2/3(T2),1/2(T3),1/3(T4)o f t h e b a s i s s y s t o l i c b l o o d p r e s s u r e,wa s c o l l e c t e d a n d ma l o n d i a l d e h y d e(MDA)、hydrogen peroxide enzyme(CAT)、glutathione oxidation reductase(GSH-P x)levels in the plasma were determined by the double antibody sandwich method of enzyme-labeled immunosorbent assay(ELISH),and the data be statistically analyzed.Results MDA:Group B less than in group C,group C less than in group D,there was no significant difference between the time level(P>0.05).CAT:Group A higher than in group B,group B higher than in group C,group C higher than in group D,there was no significant difference between the T1-T2,T2-T3,T3-T4(P>0.05),there were significant differences between the other groups(P<0.05).GSH-Px:Group A higher than in group B,group B higher than in group C,group C higher than in group D,there was no significant difference between the T1-T2(P>0.05),there were significant differences between the other groups(P<0.05).Conclusion Short term rapid blood loss has a significant effect on the antioxidant enzyme CAT and GSH-Px of organism,can lead to significantly decrease in CAT and GSH-Px activity,MDA levels rise reflect the degree of lipid oxidation.There were significantly correlated between blood loss and CAT,GSH-Px index.