云南傣族人群红细胞Rh、Duffy血型抗原多态性调查

Polymorphism of Rh and Duffy Blood Group Antigens in Erythrocytes of Dai Population in Yunnan

目的调查云南傣族人群的Rh、Duffy血型系统抗原表现型的分布及抗原频率,为云南傣族血型分布的多态性及防治Rh、Duffy血型系统引起的溶血症提供科学依据。方法采集云南省境内3代内无血缘的傣族人群200例(男女各100例)、汉族对照组100例(男女各50例)为无关人群,采集血样3 ml(EDTA抗凝),采用微柱凝胶法检测Rh、Duffy及抗体筛选,Duffy血型系统基因分型检测采用PCR-SSP法,对比分析两人群间的差异。结果 200例傣族人群中RhD阳性率为98.5%,RhD阴性率为1.5%,100例汉族人群100例(男女各50例)中RhD阳性率99.0%,RhD阴性率1.0%。Duffy基因分型检测结果:傣族200份样本中,Duffy血型各种表型分布为:Fy^((a+b-))表型187例(93.5%),Fy^((a+b+))表型12例(6.0%),Fy^((a-b+))表型1例(0.5%),未发现Fy^((a-b-))表型。基因频率为:Fy^a(0.965),Fy^b(0.035);汉族100份样本中,Duffy血型各种表型分布为:Fy^((a+b-))表型92例(92.0%),Fy^((a+b+))表型5例(5.0%),Fy^((a-b+))表型3例(3.0%),未发现Fy^((a-b-))表型。基因频率为:Fy^a(0.945),Fy^b(0.055)。傣族人群抗体筛选阳性率为1.5%,均为同种抗体,其中2例E抗体。结论云南傣族人群的Duffy血型系统抗原分布状况及抗原分布特征与当地汉族人群基本相符,但也有本民族的自身特点,傣族族人群的RhD阴性率和不规则抗体检出率比当地汉族人群的不规则抗体检出率高,差异有统计学意义(P<0.05)。

Objective To investigate the distribution and antigen frequencies of Rh and Duffy blood group antigens in Dai nationality in Yunnan,so as to provide an evidence for the prevention and treatment of blood type-associated hemolytic diseases.Methods Two hundred cases(100male and100females)of Dai people without consanguinity were collected and100Han people were taken as control.Blood samples(5ml,EDTA anticoagulants)were detected for Rh,Duffy and antibody screening.Duffy blood group genotyping was performed using PCR-SSP.Results The RhD positive rate was98.5%and the negative rate was1.5%in200Dai persons compared with the positive rate of99.0%and negative rate of1.0%in Han population.Duffy genotyping revealed the distribution of phenotypes of Fy(a+b-)(187/200,93.5%),Fy(a+b+)(12/200,6.0%),and Fy(a-b+)(1/200,0.5%),no Fy(a-b-)phenotype was seen,and the genetic frequencies were found to be Fya(0.965)and Fyb(0.035).In Han population,Duffy phenotypes include Fy(a+b-)(92/100,92.0%),Fy(a+b+)(5/100,5.0%),and Fy(a-b+)(3/100,3.0%),no Fy(a-b-)phenotype was noted,and the genetic frequencies are Fya(0.945)and Fyb(0.055).The Dai population presented the prevalence of positive antibodies of1.5%,among them2cases showed E antibodies.Duffy genotyping results showed that,in the200samples of Dai nationality,the gene frequency was Fya(0.965),and Fyb(0.035).The frequency of the gene was Fya(0.945)and Fyb(0.055)in100samples of Han nationality.Conclusion The antigen distribution of Duffy blood group system in Dai nationality is similar to those in Han population although the negative RhD and irregular antibodies are slightly higher in Dai people(P<0.05).