459例RhD阴性献血者ABO、Rh、MN和P血型基因频率的调查

Investigation on ABO,Rh,MN and P Blood Group Gene Frequency of 459 RhD Negative Blood Donors

目的探讨柳州地区RhD阴性献血者ABO、Rh、MN和P血型分布特点。方法对459例经抗球蛋白试验确认为RhD阴性的献血者,作ABO血型、Rh血型系统中的C、c、E、e抗原、MN血型系统中的M、N抗原和P血型检测,并计算各血型系统基因频率。结果 ABO血型分布:O(0.414 0)>A(0.285 4)>B(0.233 1)>AB(0.067 5);基因频率分布:r(0.645 2)>p(0.193 4)>q(0.161 4)。Rh血型分布:dccee(0.518 5)>dCcee(0.322 5)>dCCee(0.091 5)>dccEe(0.047 9)>dccEE(0.008 7)=dCcEe(0.008 7)>dCCEe(0.002 2),未检出dCcEE及dCCEE;基因频率分布:cde(0.701 1)>Cde(0.259 6)>cdE(0.039 3)>CdE(0.000 0)。MN血型分布:MN(0.568 6)>M(0.2506)>N(0.180 8);基因频率分布:M(0.534 9)>N(0.465 1)。P血型分布:P2(0.684 1)>P1(0.315 9);基因频率分布:P2(0.827 1)>P1(0.172 9)。结论柳州地区RhD阴性献血者ABO、Rh、MN和P血型分布特征总体符合中国南方人群的分布特征。

Objective To explore the distribution features of ABO,Rh,MN,and P blood group among RhD negative blood donors in Liuzhou area.Methods459RhD negative blood donors were confirmed by indirect antiglobulin test.These blood donors were detected ABO blood group,Rh(C,c,E,e)antigens,MN antigens and P blood group.The gene frequencys were calculated.Results The distribution of ABO blood group:O(0.4140)>A(0.2854)>B(0.2331)>AB(0.0675);the distribution of gene frequency:r(0.6452)>p(0.1934)>q(0.1614).The distribution of Rh blood group:dccee(0.5185)>dCcee(0.3225)>dCCee(0.0915)>dccEe(0.0479)>dccEE(0.0087)=dCcEe(0.0087)>dCCEe(0.0022),dCcEE and dCCEE could not be detected;the distribution of gene frequency:cde(0.7011)>Cde(0.2596)>cdE(0.0393)>CdE(0.0000).The distribution of MN blood group:MN(0.5686)>M(0.2506)>N(0.1808);the distribution of gene frequency:M(0.5349)>N(0.4651).The distribution of P blood group:P2(0.6841)>P1(0.3159);the distribution of gene frequency:P2(0.8271)>P1(0.1729).Co n c l u s i o n On the whole,the distribution features of ABO,Rh,MN and P blood group among RhD negative blood donors in Liuzhou area are accord with the southern Chinese population.