甲硫氨酸合酶还原酶基因A66G多态性与神经管缺陷关系的Meta分析

Association of gene polymorphism of methionine synthase reductase A66G with neural tube defectsa:ameta-analysis

目的利用Meta分析探讨母亲及子代甲硫氨酸合酶还原酶(MTRR)基因A66G多态性与神经管缺陷(NTDs)发生的相关性。方法检索万方数据库、中国知网、中国生物医学文献数据库、中文科技期刊数据库、Pub Med和Web of Science等中英文数据库,检索时间均为自建库至2015年11月。按照纳入标准选取有关母亲和(或)子代MTRR基因A66G多态性与NTDs发生相关的病例对照研究的学术期刊文献及其参考文献,提取相关数据并应用RevMan 5.0和STATA12.0软件进行Meta分析。结果 (1)有关母亲MTRR基因A66G多态性与NTDs发生相关的文献11篇,包括病例组1 284例,对照组2 182例。Meta分析结果显示,在共显性(GG基因型 vs AA基因型)及等位基因遗传模型下合并OR(95%CI)分别为1.55(1.06~2.27)、1.22(1.01~1.47),故母亲GG基因型相对AA基因型、G等位基因相对A等位基因对于子代NTDs的发生有统计学意义。(2)有关子代MTRR基因A66G多态性与NTDs发生相关的文献11篇,包括病例组1 567例,对照组2 621例。Meta分析结果显示,在共显性(AG基因型 vs AA基因型)遗传模型下合并OR(95%CI)为1.47(1.05~2.05),故子代AG基因型相对AA基因型对于NTDs的发生有统计学意义。结论母亲和子代MTRR基因A66G多态性均为NTDs发生的危险因素。

ObjectiveTo evaluate the associations between gene polymorphism of maternal and offsprings’methionine synthase reductase(MTRR)A66G and neural tube defects(NTDs)MethodsBased on inclusion criteria and retrieval strategy,the relevant literatures were identified by searches for case-control studies on the correlations between gene polymorphism of maternal and(or)offsprings’MTRR A66G with NTDs in the following databases of Wangfang,China National Knowledge Infrastructure(CNKI),China Biology Medical Literature Database(CBM),Database of Chinese Scientific and Technical Periodicals(VIP),PubMed and Web of Science from initial setup to November2015And the data were quantitatively analyzed by Rev Man50and STATA120software ResultsAmong16eligible studies,11studies(1284cases and2182controls)were on maternal MTRR A66G polymorphism and11studies(1567cases and2621controls)about offsprings’MTRR A66G polymorphism Combined OR(95%CI)on maternal MTRR A66G co-dominant model(GG vs AA)and allele(G vs A)were155(106~227)and122(101~147)respectively and those on offsprings'co dominant model(AG vs AA)were147(105~205)The results were significantly different.ConclusionBoth maternal and offsprings’MTRR gene A66G polymorphism are risk factors of NTDs