两种木腐真菌的分离及鉴定

Isolation and Identification of two Wood-rotting Fungi

在影响木材质量的众多因素中,木腐真菌侵染木材造成的木材腐朽现象对木材细胞壁破坏最为严重。为了鉴定侵染木材腐朽的病原菌,提高木材质量,本文建立一种有效鉴定木腐真菌的分子生物学方法。以采集木腐真菌子实体为研究对象,通过分离和选择培养基筛选出目的菌株,并判断木腐真菌的木材腐朽类型。运用基于PCR技术的分子生物方法,通过提取基因组DNA,primer5软件设计特异性引物对序列扩增,MEGA5.0软件构建系统发育树,对木腐真菌进行分子水平的鉴定。并结合以往研究者运用的传统形态学鉴定方法,对木腐真菌形态等特征进行观察。研究结果表明,木腐真菌S1为红缘拟层孔菌(Fomitopsis pinicola),木腐真菌S2为木蹄层孔菌(Fomes fomentarius)。将分子生物学方法与传统形态学方法相结合,能够更加准确和可靠的对木腐真菌进行鉴定。

Among all the many factors that affect the quality of wood,wood decay caused by wood-rotting fungi is the most serious damage to wood cell wall.In order to identify the pathogens causing wood decay and improve the quality of wood,a molecular biological method for the effective identification of wood-rotting fungi was established.Using collected wood-rotting fungi bodies as studying objects,the target strains were screened by separation and selection of culture medium,then the type of decayed wood-rooting fungi were determined.The DNA was extracted,and Primer5software was used to design specific primers for sequence amplification.MEGA5.0software was used to construct phylogenetic tree.And then molecular identification of wood-rotting fungi was carried out at molecular level.Combining with the traditional morphological identification methods used by previous researchers,the morphological characteristics of wood-rotting fungi were observed.The results showed that the wood-rotting fungi SI was Fomitopsis pinicola,and the wood-rotting fungi S2was Fomes fomentarius.Combining molecular biology methods with traditional morphological methods,wood-rooting fungi can be identified more accurately and reliably.